Detection of male genital infection with Chlamydia trachomatis and Neisseria gonorrhoeae using an automated multiplex PCR system (Cobas® Amplicor™)

被引:15
作者
Higgins, SP [1 ]
Klapper, PE [1 ]
Struthers, JK [1 ]
Bailey, AS [1 ]
Gough, AP [1 ]
Moore, R [1 ]
Corbitt, G [1 ]
Bhattacharyya, MN [1 ]
机构
[1] Manchester Royal Infirm, Dept Genitourinary Med, Manchester M13 9WL, Lancs, England
关键词
Chlamydia trachomatis; Neisseria gonorrhoeae; Cobas Amplicor PCR assay; urogenital samples; male;
D O I
10.1258/0956462981920982
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We evaluated Cobas(R) Amplicor(TM), a highly automated polymerase chain reaction (PCR) system, to test first-void urine (FVU) and urethral swab specimens for Chlamydia trachomatis and Neisseria gonorrhoeae in men attending a sexually transmitted infection (STI) clinic. Results were compared against an inhouse radioimmune dot blot (DB) test for C, trachomatis and selective culture for N. gonorrhoeae. Three hundred and ninety sets of specimens were obtained from 378 consecutive new and returned-new patients. Gonorrhoea prevalence was 9.49%, with no significant difference in sensitivity or specificity between culture and PCR. Chlamydia prevalence was 15.4%, with sensitivities of: DB 55%, PCR of FVU 86.7%, urethral swab PCR 90%. The specificity of PCR on FVU and urethral swabs was 100%. We have shown that Cobas(R) Amplicor(TM) PCR is highly sensitive and specific in the diagnosis of chlamydia and gonorrhoea in men attending an STI clinic. Further economic and scientific studies are needed to determine the cost-effectiveness of this technique for screening in primary care settings.
引用
收藏
页码:21 / 24
页数:4
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