Single-Molecule Spectroscopy and Imaging of Biomolecules in Living Cells

被引：120

作者：

Lord, Samuel J. ^{[1
]}

Lee, Hsiao-lu D. ^{[1
]}

Moerner, W. E. ^{[1
]}

机构：

[1] Stanford Univ, Dept Chem, Stanford, CA 94305 USA

来源：

ANALYTICAL CHEMISTRY | 2010年 / 82卷 / 06期

关键词：

LIVE CELLS; FLUORESCENT MOLECULES; PLASMA-MEMBRANE; INDIVIDUAL MOLECULES; PENTACENE MOLECULES; DIFFRACTION-LIMIT; KINESIN MOLECULES; RECEPTOR COMPLEX; LIGHT-MICROSCOPY; DNA-MOLECULES;

D O I：

10.1021/ac9024889

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

The number of reports per year on single-molecule imaging experiments has grown roughly exponentially since the first successful efforts to optically detect a single molecule were completed over two decades ago. Single-molecule spectroscopy has developed into a field that includes a wealth of experiments at room temperature and inside living cells. The fast growth of single-molecule biophysics has resulted from its benefits in probing heterogeneous populations, one molecule at a time, as well as from advances in microscopes and detectors. This Perspective summarizes die field of live-cell imaging of single biomolecules.

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页码：2192 / 2203

页数：12

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