Proteome analysis by two-dimensional gel electrophoresis and mass spectrometry: strengths and limitations

被引：121

作者：

Beranova-Giorgianni, S

机构：

[1] Univ Tennessee, Ctr Hlth Sci, Charles B Stout Neurosci Mass Spectrometry Lab, Memphis, TN 38163 USA

[2] Univ Tennessee, Ctr Hlth Sci, Dept Neurol, Memphis, TN 38163 USA

[3] Univ Tennessee, Ctr Hlth Sci, Dept Mol Sci, Memphis, TN 38163 USA

来源：

TRAC-TRENDS IN ANALYTICAL CHEMISTRY | 2003年 / 22卷 / 05期

关键词：

mass spectrometry (MS); protein identification; proteome; proteomics; two-dimensional gel electrophoresis (2DE);

D O I：

10.1016/S0165-9936(03)00508-9

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Two-dimensional gel electrophoresis, mass spectrometry and bioinformatics are the key components of current proteomics technology. The purpose of this review is to give an overview of the basic concepts of 2DE-based proteomics methodology, to discuss the advantages and limitations of the approach, and to highlight some recent advancements in 2DE-based technology. (C) 2003 Published by Elsevier Science B.V.

引用

页码：273 / +

页数：10

共 56 条

[41]

OFARRELL P, 1975, J BIOL CHEM, V50, P4007

[42]

RABILLOUD T, 2000, PROTEOME RES 2 DIMEN, P9

[43]

Santoni V, 2000, ELECTROPHORESIS, V21, P1054, DOI 10.1002/(SICI)1522-2683(20000401)21:6<1054::AID-ELPS1054>3.3.CO

[44]

2-#

[45] MALDI quadrupole time-of-flight mass spectrometry: A powerful tool for proteomic research [J].

Shevchenko, A ;

Loboda, A ;

Shevchenko, A ;

Ens, W ;

Standing, KG .

ANALYTICAL CHEMISTRY, 2000, 72 (09) :2132-2141

[46] Mass spectrometric sequencing of proteins from silver stained polyacrylamide gels [J].

Shevchenko, A ;

Wilm, M ;

Vorm, O ;

Mann, M .

ANALYTICAL CHEMISTRY, 1996, 68 (05) :850-858

[47]

Smith RD, 2001, ELECTROPHORESIS, V22, P1652, DOI 10.1002/1522-2683(200105)22:9<1652::AID-ELPS1652>3.0.CO

[48]

2-E

[49]

Tonge R, 2001, PROTEOMICS, V1, P377

[50] Difference gel electrophoresis: A single gel method for detecting changes in protein extracts [J].

Unlu, M ;

Morgan, ME ;

Minden, JS .

ELECTROPHORESIS, 1997, 18 (11) :2071-2077

← 1 2 3 4 5 6 →