The use of the 2-aminobenzoic acid tag for oligosaccharide gel electrophoresis

被引：26

作者：

Huang, ZB ^{[1
]}

Prickett, T ^{[1
]}

Potts, M ^{[1
]}

Helm, RF ^{[1
]}

机构：

[1] Virginia Tech, Fralin Biotechnol Ctr, Blacksburg, VA 24061 USA

来源：

CARBOHYDRATE RESEARCH | 2000年 / 328卷 / 01期

关键词：

anthranilic acid (2-aminobenzoic acid); gel electrophoresis; oligosaccharide; Nostoc; cyanobacteria; driselase;

D O I：

10.1016/S0008-6215(00)00045-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gel electrophoresis of fluorophore labeled saccharides provides a rapid and reliable method to screen enzymatic and/or chemical treatments of polysaccharides and glycoconjugates, as well as a sensitive and efficient microscale method to separate and purify oligosaccharides for further analysis. A simple and inexpensive method of derivatization and analysis using 2-aminobenzoic acid (anthranilic acid, AA) is described and applied to the extracellular polysaccharide released by the desiccation tolerant cyanobacterium Nostoc commune DRH-1. The results of these analyses suggest a possible protective functionality of two pendent groups, as well as a potential relationship between these groups and the desiccation tolerance of the organism. (C) 2000 Elsevier Science Ltd. All rights reserved.

引用

页码：77 / 83

页数：7

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