Overexpression of squalene-hopene cyclase by the pET vector in Escherichia coli and first identification of tryptophan and aspartic acid residues inside the QW motif as active sites

被引：31

作者：

Sato, T

Kanai, Y

Hoshino, T ^{[1
]}

机构：

[1] Niigata Univ, Fac Agr, Dept Appl Biol Chem, Niigata 95021, Japan

[2] Niigata Univ, Grad Sch Sci & Technol, Niigata 95021, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 02期

关键词：

squalene; hopene; squalene cyclase; Alicyclobacillus acidocaldarius; site-directed mutagenesis;

D O I：

10.1271/bbb.62.407

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An overexpression system for squalene-hopene cyclase (SHC) was constructed by using the pET3a vector, which is responsible for high expression with help from the strong T7 promoter when incorporated into E. coli BL21(DE3). Site-directed mutagenesis experiments prove that two amino acid residues of tryptophan and aspartic acid inside the QW-motif 5 resided as active sites.

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页码：407 / 411

页数：5

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