Carbon metabolism-mediated myogenic differentiation

被引：74

作者：

Bracha, Abigail L. ^{[2
,3
,4
]}

Ramanathan, Arvind ^{[1
]}

Huang, Sui ^{[5
]}

Ingber, Donald E. ^{[2
,3
,4
,6
,7
]}

Schreiber, Stuart L. ^{[1
,8
]}

机构：

[1] Broad Inst Harvard & MIT, Howard Hughes Med Inst, Cambridge, MA USA

[2] Childrens Hosp, Dept Pathol, Vasc Biol Program, Boston, MA 02115 USA

[3] Childrens Hosp, Dept Surg, Boston, MA 02115 USA

[4] Harvard Univ, Sch Med, Boston, MA USA

[5] Univ Calgary, Inst Biocomplex & Informat, Calgary, AB, Canada

[6] Harvard Univ, Wyss Inst Biologically Inspired Engn, Boston, MA 02115 USA

[7] Harvard Univ, Sch Engn & Appl Sci, Cambridge, MA 02138 USA

[8] Harvard Univ, Dept Chem & Chem Biol, Cambridge, MA 02138 USA

来源：

NATURE CHEMICAL BIOLOGY | 2010年 / 6卷 / 03期

基金：

美国国家科学基金会;

关键词：

HEXOSE-6-PHOSPHATE DEHYDROGENASE; STRESS;

D O I：

10.1038/NCHEMBIO.301

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The role of nutrients and metabolism in cellular differentiation is poorly understood. Using RNAi screening, metabolic profiling and small-molecule probes, we discovered that the knockdown of three metabolic enzymes-phosphoglycerate kinase (Pgk1), hexose-6-phosphate dehydrogenase (H6pd) and ATP citrate lyase (Acl)-induces differentiation of mouse C2C12 myoblasts even in the presence of mitogens. These enzymes and the pathways they regulate provide new targets for the control of myogenic differentiation in myoblasts and rhabdomyosarcoma cells.

引用

页码：202 / 204

页数：3

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