Synthesis of asymmetrically labeled sucrose by a recombinant sucrose synthase

被引：10

作者：

Nakai, T

Tonouchi, N

Tsuchida, T

Mori, H

Sakai, F

Hayashi, T

机构：

[1] KYOTO UNIV, WOOD RES INST, UJI, KYOTO 611, JAPAN

[2] BIOPOLYMER RES CO LTD, KSP, TAKATSU KU, KAWASAKI, KANAGAWA 213, JAPAN

[3] NAGOYA UNIV, FAC AGR, HIGASHIYAMA KU, NAGOYA, AICHI 46401, JAPAN

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1997年 / 61卷 / 11期

关键词：

recombinant enzyme; sucrose; asymmetrically labeling; mung bean;

D O I：

10.1271/bbb.61.1955

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

About 80% of radioactivity was recovered in asymmetrically labeled sucrose from UDP-[C-14]glucose or [C-14]fructose with recombinant mung bean sucrose synthase expressed in Escherichia coli harboring pEB-01. This high recovery is due to the fact that the enzyme conserving the activity of sucrose synthase has a similar affinity for UDP-glucose and fructose to an intact enzyme from the mung bean, but a lower affinity for sucrose.

引用

页码：1955 / 1956

页数：2

共 12 条

[1] A MEMBRANE-ASSOCIATED FORM OF SUCROSE SYNTHASE AND ITS POTENTIAL ROLE IN SYNTHESIS OF CELLULOSE AND CALLOSE IN PLANTS [J].