Dual Effect of Amino Modified Polystyrene Nanoparticles on Amyloid β Protein Fibrillation

被引：236

作者：

Cabaleiro-Lago, Celia ^{[1
]}

Quinlan-Pluck, Fiona ^{[1
]}

Lynch, Iseult ^{[1
]}

Dawson, Kenneth A. ^{[1
]}

Linse, Sara ^{[2
]}

机构：

[1] Natl Univ Ireland Univ Coll Dublin, Sch Chem & Chem Biol, Ctr BioNano Interact, Dublin 4, Ireland

[2] Lund Univ, Dept Biochem, S-22100 Lund, Sweden

来源：

ACS CHEMICAL NEUROSCIENCE | 2010年 / 1卷 / 04期

基金：

瑞典研究理事会; 爱尔兰科学基金会;

关键词：

Amyloid; aggregation kinetics; nanoparticles; ALZHEIMERS-DISEASE; AGGREGATION; NUCLEATION; INHIBITION; KINETICS;

D O I：

10.1021/cn900027u

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The fibrillation kinetics of the amyloid beta peptide is analyzed in presence of cationic polystyrene nanoparticles of different size. The results highlight the importance of the ratio between the peptide and particle concentration. Depending on the specific ratio, the kinetic effects vary from acceleration of the fibrillation process by reducing the lag phase at low particle surface area in solution to inhibition of the fibrillation process at high particle surface area. The kinetic behavior can be explained if we assume a balance between two different pathways: first fibrillation of free monomer in solution and second nucleation and fibrillation promoted at the particle surface. The overall rate of fibrillation will depend on the interplay between these two pathways, and the predominance of one mechanism over the other will be determined by the relative equilibrium and rate constants.

引用

页码：279 / 287

页数：9

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