Acupuncture decreases ischemia-induced apoptosis and cell proliferation in dentate gyrus of gerbils

Background: Acupuncture has been used for the enhancement of functional recovery from various disorders. In the present study, the effect of acupuncture on the apoptosis and new cell proliferation in the hippocampal dentate gyrus of gerbils (n=25) following transient global ischemia was investigated. Methods: To determine the level of apoptosis and cell proliferation, terminal deoxynucleotidyl transferase-mediated dUTP nick end-labeling (TUNEL) assay and immunohistochemistry for 5-bromo-2'-deoxyuridine (BrdU) were employed respectively. Results: In the dentate gyrus of ischemic gerbils, the number of both TUNEL- and BrdU-positive cells (66.01 +/- 2.45/mm(2) and 514.38 +/- 44. 90/mm(2)) was significantly increased compared to that of the sGam-operated gerbils (11.25 +/- 1.85/mm(2) and 117.47 +/- 10.95/mm(2)). Among the acupuncture (ST36, L14 or non-acupoint) treated groups, ST36 acupoint treated group showed the most potent apoptosis (20.52 +/- 2.40/mm(2)) and proliferation (159.38 +/- 24.05/mm(2)) suppressive effects (similar to 70% decreases in both apoptosis and cell proliferation). Conclusion: These results may suggest that acupuncture treatment alleviates ischemia-induced apoptosis and presents possible therapeutic potentials in the recovery from ischemic cerebral injury.