A novel ring-like complex of Xenopus proteins essential for the initiation of DNA replication

被引：165

作者：

Kubota, Y

Takase, Y

Komori, Y

Hashimoto, Y

Arata, T

Kamimura, Y

Araki, H

Takisawa, H ^{[1
]}

机构：

[1] Osaka Univ, Grad Sch Sci, Dept Biol, Toyonaka, Osaka 5600043, Japan

[2] Grad Univ Adv Studies, Shizuoka 4118540, Japan

[3] Natl Inst Genet, Div Microbial Genet, Shizuoka 4118540, Japan

来源：

GENES & DEVELOPMENT | 2003年 / 17卷 / 09期

关键词：

DNA replication; initiation complex; MCM; Cdc45; S-phase CDK;

D O I：

10.1101/gad.1070003

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We have identified Xenopus homologs of the budding yeast Sld5 and its three interacting proteins. These form a novel complex essential for the initiation of DNA replication in Xenopus egg extracts. The complex binds to chromatin in a manner dependent on replication licensing and S-phase CDK. The chromatin binding of the complex and that of Cdc45 are mutually dependent and both bindings require Xenopus Cut5, the yeast homolog of which interacts with Sld5. On replicating chromatin the complex interacts with Cdc45 and MCM, putative components of replication machinery. Electron microscopy further reveals that the complex has a ring-like structure. These results suggest that the complex plays an essential role in the elongation stage of DNA replication as well as the initiation stage.

引用

页码：1141 / 1152

页数：12

共 44 条

[41] Xenopus Mcm10 binds to origins of DNA replication after Mcm2-7 and stimulates origin binding of Cdc45 [J].