The comparative analysis of osmotins and osmotin-like PR-5 proteins

被引：57

作者：

Anzlovar, S ^{[1
]}

Dermastia, M ^{[1
]}

机构：

[1] Univ Ljubljana, Dept Biol, Biotech Fac, Ljubljana 1000, Slovenia

来源：

PLANT BIOLOGY | 2003年 / 5卷 / 02期

关键词：

PR-5; structure; gene expression; mechanism of antifungal activity;

D O I：

10.1055/s-2003-40723

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

One of the ways that plants respond to biotic and/or abiotic stress factors is the accumulation of pathogenesis-related proteins of class 5 (PR-5), which are evolutionary conserved in the plant kingdom. Within the PR-5 family, a distinct subgroup of osmotin and closely related proteins has been characterized. In contrast to the extracellular forms of PR-5 proteins, osmotins presumably accumulate in the vacuole of the cell. They contain a C-terminal propeptide that is considered to be a determinant for vacuolar targeting. The comparison of the three-dimensional structure of tobacco IPR-5 d with the sequences of some osmotins showed that the proteins consist of three conserved domains, with the acidic cleft between domains I and II. Besides the constitutive species and tissue-specific presence, the osmotins are also induced by several abiotic and biotic stresses. Among them, fungal infections can elicit osmotin gene expression, and most known proteins from the family have antifungal activity in in vitro assays. In agreement with the osmotin structure and data on the activity of similar proteins, a two-step mechanism, which involves reaction of osmotins with the fungal wall and the permeabilization of fungal membranes, is discussed.

引用

页码：116 / 124

页数：9

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