Single-molecule conformational analysis of G-quadruplex formation in the promoter DNA duplex of the proto-oncogene C-kit

被引：109

作者：

Shirude, Pravin S.

Okumus, Burak

Ying, Liming

Ha, Taekjip

Balasubramanian, Shankar

机构：

[1] Univ Cambridge, Dept Chem, Cambridge CB2 1EW, England

[2] Univ Illinois, Howard Hughes Med Inst, Dept Phys, Urbana, IL 61801 USA

[3] Imperial Coll London, Natl Heart & Lung Inst, Biol Nanosci Sect, London SW7 2AZ, England

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2007年 / 129卷 / 24期

基金：

英国生物技术与生命科学研究理事会;

关键词：

D O I：

10.1021/ja070497d

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Herein we report on the single-molecule analysis of a conserved DNA quadruplex element found in the transcriptional activation site of the promoter of the proto- oncogene c-kit. We demonstrate that the c-kit quadruplex motif is able to fold into nonduplex states within a natural extended DNA duplex. There are apparent differences in putative quadruplex structures, and the K+ dependence on quadruplex formation, that appear to result when studying this quadruplex motif in natural duplex, as opposed to the single-stranded form.

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收藏

页码：7484 / +

页数：3

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