Analysis of multiple constituents in a Chinese herbal preparation Shuang-Huang-Lian oral liquid by HPLC-DAD-ESI-MSn

被引：152

作者：

Han, Jian ^{[1
]}

Ye, Min ^{[1
]}

Guo, Hui ^{[1
]}

Yang, Min ^{[1
]}

Wang, Bao-rong ^{[1
]}

Guo, De-an ^{[1
]}

机构：

[1] Peking Univ, Sch Pharmaceut Sci, Beijing 100871, Peoples R China

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 2007年 / 44卷 / 02期

关键词：

HPLC-DAD-ESI-MSn; multiple constituents; Chinese herbal preparation; Shuang-Huang-Lian oral liquid;

D O I：

10.1016/j.jpba.2007.02.023

中图分类号：

O65 [分析化学];

学科分类号：

070302 [分析化学]; 081704 [应用化学];

摘要：

A high-performance liquid chromatography/mass spectrometry (HPLC-MS) method for the quality control of Shuang-Huang-Lian oral liquid, an antimicrobial and antipyretic herbal preparation, has been developed. Pure compounds are subjected to tandem mass spectrometry (MSn) analysis to clarify their fragmentation rules. Then, the sample of Shuang-Huang-Lian was analyzed by on-line LC-MSn. A total of 27 compounds, including seven phenylethanoid glycosides, three lignans, seven quinic acids, six saponins and four flavonoids, in the extract of Shuang-Huang-Lian oral liquid have been identified or tentatively characterized. It is expected to develop a comprehensive quality control method of this commonly used herbal preparation. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：430 / 438

页数：9

共 30 条

[1]

Development of the chromatographic fingerprint of herbal preparations Shuang-Huang-Lian oral liquid [J].