Cryopreservation of oocytes from pre-antral follicles

被引:47
作者
Amorim, CA
Gonçalves, PBD
Figueiredo, JR
机构
[1] Univ Fed Santa Maria, Ctr Ciencias Rurais, Programa Posgrad Med Vet, BR-97105900 Santa Maria, RS, Brazil
[2] Univ Estadual Ceara, Fac Vet, Dept Vet Med, BR-60740000 Fortaleza, Ceara, Brazil
关键词
cryoprotectant; freezing; isolated follicles; oocyte; ovarian tissue;
D O I
10.1093/humupd/dmg014
中图分类号
R71 [妇产科学];
学科分类号
100211 ;
摘要
Cryobiology is a very important tool in reproductive biology. Research in this area focuses on the possibility of restoring fertility in women with reproductive problems or after cancer treatments. Another goal is to establish a genetic resource bank for endangered or commercially important animal species. Cryopreservation of oocytes from pre-antral follicles has been studied during the past decade. Procedures can be divided between the cryopreservation of either ovarian tissue or isolated follicles. Most studies describe a slow freezing/rapid thawing protocol to cryopreserve ovarian fragments. Histology shows that the follicles maintain their morphological integrity, and transplantation of ovarian tissue demonstrates that the follicles can restart their growth and eventually ovulate. Some research groups have obtained offspring using this procedure in mice and sheep. With regard to the cryopreservation of isolated follicles, the few studies reported in this area used the same freezing protocol, and some of them described follicular growth using in-vitro culture. The best result was obtained in mice, with animal birth after follicular cryopreservation and culture. However, additional studies are necessary for a better understanding of the events during follicular cryopreservation and to establish a standard protocol for ovarian transplantation or follicle culture.
引用
收藏
页码:119 / 129
页数:11
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