Immobilization of Rhodococcus AJ270 and use of entrapped biocatalyst for the production of acrylic acid

被引：25

作者：

Colby, J ^{[1
]}

Snell, D ^{[1
]}

Black, GW ^{[1
]}

机构：

[1] Univ Sunderland, Sch Sci, Sunderland SR1 3SD, Durham, England

来源：

MONATSHEFTE FUR CHEMIE | 2000年 / 131卷 / 06期

关键词：

acrylamide; amidase; acrylate; biocatalysis; Rhodococcus;

D O I：

10.1007/s007060070094

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Rhodococcus AJ270 is adsorbed by Dowex 1 at 15.4 mg dry weight per g resin with maximum amidase specific activity observed at lower loadings. Bacteria form a monolayer on the resin surface, and adsorption is complete within 2 min. AJ270 can be entrapped in agar and agarose gels (optimum loading: 20 mg dry weight bacteria per cm(3) gel). Adsorption and entrapment improve amidase thermal stability 3-4 fold, and entrapment shifts the pH optimum from 8 to 7. Adsorbed and free bacteria show similar values for K-m and V-max, but entrapped bacteria have higher K-m values. Compared with bacteria adsorbed to Dowex, the activity per cm3 of matrix of agar-entrapped AJ270 is eight-fold higher. In stirred-tank reactors, exposure to acrylic acid reduces the amidase activity of the biocatalyst in the hydrolysis of acrylamide. In column reactors, entrapped AJ270 suffers Little reduction in amidase activity against 0.25 M acrylamide over 22 h continuous operation.

引用

页码：655 / 666

页数：12

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