Optical Thermophoresis for Quantifying the Buffer Dependence of Aptamer Binding

被引：175

作者：

Baaske, Philipp ^{[1
,2
]}

Wienken, Christoph J. ^{[1
]}

Reineck, Philipp ^{[1
]}

Duhr, Stefan ^{[2
]}

Braun, Dieter ^{[1
]}

机构：

[1] Univ Munich, Dept Phys, Ctr NanoSci CeNS, D-80799 Munich, Germany

[2] NanoTemper Technol GmbH, D-80799 Munich, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2010年 / 49卷 / 12期

关键词：

aptamers; DNA; drug discovery; immobilization-free methods; proteins; SINGLE-STRANDED-DNA; MOLECULAR RECOGNITION; CHARGED COLLOIDS; SELECTION; THERMODIFFUSION; FLUORESCENCE; DIFFUSION; ADENOSINE; INVITRO; LIGANDS;

D O I：

10.1002/anie.200903998

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

"Chemical Equation Presented" Some like it hot: A robust and fast method for characterizing aptamers relies on the distinct thermophoretic movements of molecules in microscopic temperature gradients (see diagram). The binding properties of proteins and even small molecules can be measured within seconds, and less than 1 μL of sample is required. Notably, the technique works well in complex liquids such as human serum. © 2010 Wiley-VCH Verlag GmbH & Co. KCaA.

引用

页码：2238 / 2241

页数：4

共 28 条

[1]

[Anonymous], 2009, ANGEW CHEM, V121, P2710

[2] Melting curve analysis in a snapshot [J].

Baaske, Philipp ;

Duhr, Stefan ;

Braun, Dieter .

APPLIED PHYSICS LETTERS, 2007, 91 (13)

[3] Design Variations for an Aptamer-Based DNA Nanodevice [J].

Beyer, Stefan ;

Dittmer, Wendy U. ;

Simmel, Friedrich C. .

JOURNAL OF BIOMEDICAL NANOTECHNOLOGY, 2005, 1 (01) :96-101

[4] SELECTION OF SINGLE-STRANDED-DNA MOLECULES THAT BIND AND INHIBIT HUMAN THROMBIN [J].

BOCK, LC ;

GRIFFIN, LC ;

LATHAM, JA ;

VERMAAS, EH ;

TOOLE, JJ .

NATURE, 1992, 355 (6360) :564-566

[5] In vitro selection and characterization of cellulose-binding RNA aptamers using isothermal amplification [J].

Boese, B. J. ;

Corbino, K. ;

Breaker, R. R. .

NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS, 2008, 27 (08) :949-966

[6] Dependence of aptamer activity on opposed terminal extensions: improvement of light-regulation efficiency [J].

Buff, Maximilian C. R. ;

Schäfer, Florian ;

Wulffen, Bernhard ;

Mueller, Jens ;

Potzsch, Bernd ;

Heckel, Alexander ;

Mayer, Gunter .

NUCLEIC ACIDS RESEARCH, 2010, 38 (06) :2111-2118

[7] Applications of Aptamers as Sensors [J].

Cho, Eun Jeong ;

Lee, Joo-Woon ;

Ellington, Andrew D. .

ANNUAL REVIEW OF ANALYTICAL CHEMISTRY, 2009, 2 :241-264

[8] Thermodiffusion of charged colloids: Single-particle diffusion [J].

Dhont, Jan K. G. ;

Wiegand, S. ;

Duhr, S. ;

Braun, D. .

LANGMUIR, 2007, 23 (04) :1674-1683

[9] Thermodiffusion of interacting colloids. I. A statistical thermodynamics approach [J].

Dhont, JKG .

JOURNAL OF CHEMICAL PHYSICS, 2004, 120 (03) :1632-1641

[10] Thermophoresis of DNA determined by microfluidic fluorescence [J].

Duhr, S ;

Arduini, S ;

Braun, D .

EUROPEAN PHYSICAL JOURNAL E, 2004, 15 (03) :277-286

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