Murine filariasis: interleukin 4 and interleukin 5 lead to containment of different worm developmental stages

被引:80
作者
Volkmann, L
Bain, O
Saeftel, M
Specht, S
Fischer, K
Brombacher, F
Matthaei, KI
Hoerauf, A
机构
[1] Bernhard Nocht Inst Trop Med, D-20359 Hamburg, Germany
[2] Museum Natl Hist Nat, CNRS, Inst Systemat, Biol Parasitaire FR 1541, Paris, France
[3] Univ Cape Town, Groote Schuur Hosp, Hlth Fac, Dept Immunol, ZA-7925 Cape Town, South Africa
[4] Australian Natl Univ, John Curtin Sch Med Res, Div Biochem & Mol Biol, Canberra, ACT 2601, Australia
关键词
interleukin-4; interleukin-5; murine filariasis; neutrophils; eosinophils;
D O I
10.1007/S00430-002-0155-9
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
We compared the impact of IL-4 and IL-5 deficiency during the fully permissive infection of BALB/c mice with the rodent filaria Litomosoides sigmodontis. IL-5, in contrast to IL-4, is crucial for the containment of adult worms during short- and long-term infections. IL-5 KO mice allowed development of more larvae into adult worms and showed up to 200 times more adult worms persisting during chronic infection (day 60 until 200 post-infection). This increased persistence was accompanied by a reduction in inflammatory nodules around adult filariae. In contrast, adult worm survival and nodule formation did not differ between BALB/c wild-type mice and BALB/c IL-4 KO or BALB/c IL-4 receptor (IL-4R) alpha-chain KO mice. In both IL-4 and IL-5 KO mice microfilaraemia was greatly enhanced (160-fold) and prolonged compared to wild-type mice. This extent of susceptibility to microfilariae required the presence of adult worms in a full infection cycle since upon intraperitoneal injection of microfilariae alone they were removed from BALB/c, BALB/c IL-4 KO and BALB/c IL-4R alpha-chain KO mice with equivalent kinetics, and since microfilarial survival was only slightly increased in IL-5 KO mice (factor of 5 vs. factor of 160 in full infection). In conclusion, IL-4 and IL-5 dependent effector pathways operate against different stages of filarial worms, and IL-5 has a greater impact on parasite containment than IL-4.
引用
收藏
页码:23 / 31
页数:9
相关论文
共 40 条
[1]   The Xid defect imparts susceptibility to experimental murine filariosis - association with a lack of antibody and IL-10 production by B cells in response to phosphorylcholine [J].
Al-Qaoud, KM ;
Fleischer, B ;
Hoerauf, A .
INTERNATIONAL IMMUNOLOGY, 1998, 10 (01) :17-25
[2]   A new mechanism for IL-5-dependent helminth control: neutrophil accumulation and neutrophil-mediated worm encapsulation in murine filariasis are abolished in the absence of IL-5 [J].
Al-Qaoud, KM ;
Pearlman, E ;
Hartung, T ;
Klukowski, J ;
Fleischer, B ;
Hoerauf, A .
INTERNATIONAL IMMUNOLOGY, 2000, 12 (06) :899-908
[3]   Infection of BALB/c mice with the filarial nematode Litomosoides sigmodontis: Role of CD4(+) T cells in controlling larval development [J].
AlQaoud, KM ;
Taubert, A ;
Zahner, H ;
Fleischer, B ;
Hoerauf, A .
INFECTION AND IMMUNITY, 1997, 65 (06) :2457-2461
[4]   LARVAL BIOLOGY OF 6 FILARIAE OF THE SUBFAMILY ONCHOCERCINAE IN A VERTEBRATE HOST [J].
BAIN, O ;
WANJI, S ;
VUONG, PN ;
MARECHAL, P ;
LEGOFF, L ;
PETIT, G .
PARASITE, 1994, 1 (03) :241-254
[5]  
BANCROFT AJ, 1993, J IMMUNOL, V150, P1395
[6]   Neutrophil accumulation around Onchocerca worms and chemotaxis of neutrophils are dependent on Wolbachia endobacteria [J].
Brattig, NW ;
Büttner, DW ;
Hoerauf, A .
MICROBES AND INFECTION, 2001, 3 (06) :439-446
[7]  
Brombacher F, 2000, BIOESSAYS, V22, P646, DOI 10.1002/1521-1878(200007)22:7&lt
[8]  
646::AID-BIES7&gt
[9]  
3.0.CO
[10]  
2-9