Cytokine profile induced by a primary infection with Ostertagia ostertagi in cattle

被引:44
作者
Canals, A
Zarlenga, DS
Almeria, S
Gasbarre, LC
机构
[1] USDA, ARS, LPSI, Immunol. and Dis. Resist. Laboratory, Bldg. 1180, USDA, Beltsville
关键词
Ostertagia ostertagi; immunity; bovine; cytokines;
D O I
10.1016/S0165-2427(96)05775-3
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Changes that occur in the local draining lymph nodes including, changes in cell surface markers and cytokine gene expression were studied over the first 4 weeks of a primary, Ostertagia ostertagi infection of the abomasum. Cells recovered from the abomasal lymph nodes (ABLN) after infection showed a decrease in the percentage of CD3(+) cells, and an increase in the percentage of IgM(+) cells and cells bearing the TcR1 marker. These changes were coincident with an increase in the proportion of activated cells (Il-2R). Analysis of mitogen-stimulated ABLN cells by RNase protection assay (RPA) showed a dramatic reduction in IL-2 and IFN-gamma transcription after infection. In addition, analysis of unstimulated ABLN cells by competitive RT-PCR showed a similar decrease in demonstrable levels of IL-2 mRNA, but IL-10, IL-4 and IFN-gamma mRNA levels were elevated. (C) 1997 Elsevier Science B.V.
引用
收藏
页码:63 / 75
页数:13
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