Development of sensitive crop-specific polymerase chain reaction assays using 5S DNA: Applications in food traceability

被引：23

作者：

Doveri, Silvia ^{[1
]}

Lee, David ^{[1
]}

机构：

[1] NIAB, Crop Genet Grp, Cambridge CB3 0LE, England

来源：

JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | 2007年 / 55卷 / 12期

关键词：

DNA detection; soy; sunflower; maize; oilseed rape; OLIVE OIL; RNA GENES; EXTRACTION; SEQUENCE; PCR; EVOLUTION; PISUM; MAIZE;

D O I：

10.1021/jf063259v

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

The 5S intergenic spacers were amplified using a common pair of primers and sequenced from four species (Brassica napus, Zea mays, Helianthus annuus, and Glycine max). Crop-specific assays were developed from primers designed from the spacers and tested to amplify corresponding DNAs in both conventional end-point and real-time polymerase chain reactions (PCRs). The high copy numbers of the 5S DNA in plants make it possible to detect very small amounts of DNA using this marker. This sensitivity made it possible to compare different DNA extraction methods for highly processed food products using 5S spacers, even allowing dilution of templates to overcome PCR inhibition.

引用

收藏

页码：4640 / 4644

页数：5

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