HLA-G in reproduction: Studies on the maternal-fetal interface

被引:59
作者
Hunt, JS
Petroff, MG
Morales, P
Sedlmayr, P
Geraghty, DE
Ober, C
机构
[1] Univ Kansas, Med Ctr, Dept Anat & Cell Biol, Kansas City, KS 66160 USA
[2] Univ Kansas, Med Ctr, Dept Pathol & Lab Med, Kansas City, KS 66103 USA
[3] Karl Franzens Univ Graz, Inst Histol & Embryol, Graz, Austria
[4] Fred Hutchinson Canc Res Ctr, Human Genet Program, Seattle, WA 98104 USA
[5] Univ Chicago, Dept Human Genet, Chicago, IL 60637 USA
关键词
HLA-G; immunoglobulinlike transcript (ILT); placenta; macrophage; trophoblast;
D O I
10.1016/S0198-8859(00)00195-6
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
For more chan a decade, investigators have known that membrane-bound and soluble isoforms of the HLA class Ii molecule, HLA-G, are present at the maternal-fetal interface. Although it is clear that extravillous cytotrophoblast cells are major producers, other cells may also contribute. Recent studies in our laboratory raised the question of whether soluble isoforms might reach the maternal and/or fetal blood circulation. A capture enzyme-linked immunoabsorbent assay (ELISA) identified soluble HLA-G (sHLA-G) in maternal blood throughout pregnancy tut failed to detect sHLA-G in cord sera. Further studies suggested that the circulating proteins may be either free heavy chain (sHLA-G1 and/or sHLA-G2) or exclusively sHLA-G2. To study the potential function(s) of the soluble isoforms to modulate local or systemic immunity in mothers, we generated recombinant sHLA-G1 and -G2 in both prokaryotic and eukaryotic systems. Preliminary experiments conducted using DNA microarray analysis suggest that sHLA-G is capable of modulating gene expression in blood mononuclear leukocytes. Potential local targets were also identified; decidual and placental macrophages but not trophoblast cells contained mRNA encoding two of the known receptors for HLA-G, ILT2 and ILT4. Collectively, the studies are consistent with the hypothesis that sHLA-G produced at the maternal-fetal interface targets to the cells of the monocyte/macrophage lineage and modulates their functions for the benefit of pregnancy. (C) American Society for Histocompatibility and Immunogenetics, 2000. Published by Elsevier Science Inc.
引用
收藏
页码:1113 / 1117
页数:5
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