Contribution of androgens to the gender difference in leptin production in obese children and adolescents

被引:304
作者
Wabitsch, M
Blum, WF
Muche, R
Braun, M
Hube, F
Rascher, W
Heinze, E
Teller, W
Hauner, H
机构
[1] UNIV ULM,DEPT BIOMETRY & MED DOCUMENTAT,D-89075 ULM,GERMANY
[2] UNIV GIESSEN,DEPT PEDIAT,D-35392 GIESSEN,GERMANY
[3] LILLY DEUTSCHLAND,D-61350 HOMBURG,GERMANY
[4] DIABET RES INST,D-40225 DUSSELDORF,GERMANY
关键词
androgens; gender difference; human adipose tissue; leptin;
D O I
10.1172/JCI119595
中图分类号
R-3 [医学研究方法]; R3 [基础医学];
学科分类号
1001 ;
摘要
Recent studies demonstrated significantly higher serum leptin concentrations in females as compared with males, even after correction for differences in body fat mass. The aim of our study was to measure serum leptin concentrations in a large group of obese children and adolescents to determine the possible role of sea steroid hormones on both leptin serum concentrations and production in human adipocytes. Obese girls were found to have significantly higher leptin concentrations than boys at the same degree of adiposity (25.2+/-14.1 vs. 17.2+/-12.6 ng/ml, P < 0.001). Ln a multiple regression analysis with age and body mass index (percent body fat) as fixed variables, it turned out that testosterone had a potent negative effect on serum leptin in boys, but not in girls. Ln vitro experiments using newly developed human adipocytes in primary culture showed that both testosterone and its biologically active metabolite dihydrotestosterone are able to reduce leptin secretion into the culture medium by up to 62%, Using a semiquantitative reverse transcriptase-PCR method, testosterone was found to suppress leptin mRNA to a similar extent. These results suggest that, apart from differences in body fat mass, the higher androgen concentrations in obese boys are responsible fur the lower leptin serum concentrations compared with obese girls.
引用
收藏
页码:808 / 813
页数:6
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