Antioxidant hydrocolloids from herb Graptopetalum paraguayense leaves show anti-colon cancer cells and anti-neuroinflammatory potentials

被引：8

作者：

Ai, Lianzhong ^{[1
]}

Chung, Yun-Chin ^{[2
]}

Jeng, Kee-Ching G. ^{[3
]}

Lai, Phoency F. -H. ^{[1
,2
,4
]}

Yeh, Shu-Chen ^{[2
]}

Lee, Kuan Ching ^{[2
]}

Lin, Syuan-Yu ^{[2
]}

Xia, Yongjun ^{[1
]}

Wang, Guangqiang ^{[1
]}

Cui, Steve W. ^{[5
]}

机构：

[1] Univ Shanghai Sci & Technol, Shanghai Engn Res Ctr Food Microbiol, Sch Med Instrument & Food Engn, Shanghai 200093, Peoples R China

[2] Providence Univ, Dept Food & Nutr, Taichung 43301, Taiwan

[3] Taichung Vet Gen Hosp, Dept Educ & Res, Taichung 40705, Taiwan

[4] Conpowon Int Co, MediFood Res Ctr, New Taipei 25170, Taiwan

[5] Agr & Agri Food Canada, Guelph Food Res Ctr, Guelph, ON N1G 5C9, Canada

来源：

FOOD HYDROCOLLOIDS | 2017年 / 73卷

关键词：

Graptopetalum; Antioxidant; Anti-cancer cell; Anti-inflammation; Human colon adenocarcinoma Caco-2; Microglia BV-2; SUGAR-BEET PECTIN; E; WALTHER; PROLIFERATION; EXTRACTS; CACO-2; ASSAY;

D O I：

10.1016/j.foodhyd.2017.06.027

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Leaves of herb Graptopetalum paraguayense E. Walther are considered to be applied for functional drinks or isolated for nutraceutical ingredients. This study was to preliminarily elucidate the antioxidant activities and potentials for anti-colon cancer cells and anti-neuroinflammation of the hot-water extracts and its macromolecular hydrocolloids from freeze-dried G. paraguayense leaves, in relation to detectable polysaccharide compositions and associated phenolic and non-phenolic compounds. It is indicated that the 100 degrees C-water extract (GW100) form G. paraguayense leaves contained glucans at a molar ratio of glucose (Glc): fucose (Fuc): xylose (Xyl): ribose (Rib) = 1: 0.29: 0.13: 0.13 and a major macromolecular species with an averaged molecular weight (M-w) = 2172. The macromolecular GW100E, i.e. 72%-ethanol precipitate of GW100, contained pectin substance like arabino-rhamnogalactans at a molar ratio of galactose (Gal): arabinose (Ara): rhamnose (Rha): Glc = 1: 0.81: 0.34: 0.32, and only one macromolecular species with an averaged M-w = 2405. Both GW100 and GW100E exhibited excellent antioxidant activities in scavenging alpha, alpha-diphenyl-beta-picrylhydrazyl radicals (DPPH center dot) ,2,2-azino-bis [3-ethylbenzthiazoline-6-sulfonic acid] radicals (ABTS(+)), and superoxide anions (O-2(-)), great potential in anti-proliferation against human colon adenocarcinoma Caco-2 cells, and significant immunomodulatory activities on murine microglia BV-2 cells. Comparatively, GW100 was outstanding in scavenging ABTS(+) and O-2(-) and inhibiting significantly tumor necrosis factor-alpha (TNF-alpha) and interleukine-6 (IL-6) production by lipopolysaccharide (LPS)-treated BV-2 cells. GW100E excelled in scavenging DPPH, and anti-Caco-2 proliferation. Conclusively, GW100 and GW100E could be good candidates for anti-neuro-inflammation potentials and anti-Caco-2 proliferation, respectively. (C) 2017 Elsevier Ltd. All rights reserved.

引用

页码：51 / 59

页数：9

共 39 条

[1]

Synthesis and characterization of pectin derivative with antitumor property against Caco-2 colon cancer cells [J].