Plasma membrane localization and fusion inhibitory activity of the cowpox virus serpin SPI-3 require a functional signal sequence and the virus encoded hemagglutinin

被引:24
作者
Brum, LM [1 ]
Turner, PC [1 ]
Devick, H [1 ]
Baquero, MT [1 ]
Moyer, RW [1 ]
机构
[1] Univ Florida, Coll Med, Dept Mol Genet & Microbiol, Gainesville, FL 32610 USA
关键词
poxvirus; serpin; SPI-3; HA; fusion; localization; EEV;
D O I
10.1016/S0042-6822(02)00017-X
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The cowpox virus (CPV) glycoprotein serpin SPI-3, a functional protease inhibitor, and the viral hemagglutinin (HA) are required to prevent fusion of wt CPV infected cells. SPI-3 and HA from CPV infected cells co-localize to the plasma membrane and are found in extracellular enveloped virus (EEV). We also show that an N-terminal SPI-3 signal sequence, but not glycosylation, is required for membrane localization and fusion inhibition. In the absence of HA (CPVDeltaHA), no SPI-3 is found on the membrane and infected cells fuse. Conversely, HA from both wt CPV and CPVDeltaSPI-3 infections is on the membrane, indicating a requirement of HA for SPI-3 plasma membrane localization. In the absence of HA, secretion of SPI-3 or SPI-3 N-glyc(-) was markedly enhanced, suggesting HA serves to retain SPI-3 on the plasma membrane,thereby preventing cell fusion. (C) 2003 Elsevier Science (USA). All rights reserved.
引用
收藏
页码:289 / 302
页数:14
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