Cryoelectron microscopic examination of human immunodeficiency virus type 1 virions with mutations in the cyclophilin A binding loop

被引：22

作者：

Kong, LB

An, DS

Ackerson, B

Canon, J

Rey, O

Chen, ISY

Krogstad, P

Stewart, PL

机构：

[1] Univ Calif Los Angeles, Sch Med, Dept Mol & Med Pharmacol, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Sch Med, Crump Inst Biol Imaging, Los Angeles, CA 90095 USA

[3] Univ Calif Los Angeles, Sch Med, Dept Microbiol & Immunol, Los Angeles, CA 90095 USA

[4] Univ Calif Los Angeles, Sch Med, Dept Med, Los Angeles, CA 90095 USA

[5] Univ Calif Los Angeles, Sch Med, Dept Pediat, Los Angeles, CA 90095 USA

来源：

JOURNAL OF VIROLOGY | 1998年 / 72卷 / 05期

关键词：

D O I：

10.1128/JVI.72.5.4403-4407.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The human immunodeficiency virus type 1 capsid protein contains a conserved P217X4PX2PX5P231 motif. Mutation at Pro-222 decreases virion incorporation of cyclophilin A, while mutation at Pro-231 abolishes infectivity. Although viral RNA incorporation and protease cleavage of the Gag precursor were not affected by these mutations, cryoelectron microscopy revealed a loss of virion maturation in P231A particles.

引用

收藏

页码：4403 / 4407

页数：5

相关论文

共 44 条

[1] Cells with high cyclophilin A content support replication of human immunodeficiency virus type 1 Gag mutants with decreased ability to incorporate cyclophilin A [J].

Ackerson, B ;

Rey, O ;

Canon, J ;

Krogstad, P .

JOURNAL OF VIROLOGY, 1998, 72 (01) :303-308

[2] PRODUCTION OF ACQUIRED IMMUNODEFICIENCY SYNDROME-ASSOCIATED RETROVIRUS IN HUMAN AND NONHUMAN CELLS TRANSFECTED WITH AN INFECTIOUS MOLECULAR CLONE [J].

ADACHI, A ;

GENDELMAN, HE ;

KOENIG, S ;

FOLKS, T ;

WILLEY, R ;

RABSON, A ;

MARTIN, MA .

JOURNAL OF VIROLOGY, 1986, 59 (02) :284-291

[3] CRYO-ELECTRON MICROSCOPY OF VIRUSES [J].

ADRIAN, M ;

DUBOCHET, J ;

LEPAULT, J ;

MCDOWALL, AW .

NATURE, 1984, 308 (5954) :32-36

[4] Cyclophilin A-induced alterations of human immunodeficiency virus type 1 CA protein in vitro [J].

Agresta, BE ;

Carter, CA .

JOURNAL OF VIROLOGY, 1997, 71 (09) :6921-6927

[5] High-efficiency transduction of human lymphoid progenitor cells and expression in differentiated T cells [J].

An, DS ;

Koyanagi, Y ;

Zhao, JQ ;

Akkina, R ;

Bristol, G ;

Yamamoto, N ;

Zack, JA ;

Chen, ISY .

JOURNAL OF VIROLOGY, 1997, 71 (02) :1397-1404

[6] Determination of the fold of the core protein of hepatitis B virus ky electron cryomicroscopy [J].

Bottcher, B ;

Wynne, SA ;

Crowther, RA .

NATURE, 1997, 386 (6620) :88-91

[7] Cyclophilin A is required for an early step in the life cycle of human immunodeficiency virus type 1 before the initiation of reverse transcription [J].

Braaten, D ;

Franke, EK ;

Luban, J .

JOURNAL OF VIROLOGY, 1996, 70 (06) :3551-3560

[8] The hydrophobic pocket of cyclophilin is the binding site for the human immunodeficiency virus type 1 Gag polyprotein [J].

Braaten, D ;

Ansari, H ;

Luban, J .

JOURNAL OF VIROLOGY, 1997, 71 (03) :2107-2113

[9]

Canon J, 1996, BIOTECHNIQUES, V20, P878

[10] Visualization of a 4-helix bundle in the hepatitis B virus capsid by cryo-electron microscopy [J].

Conway, JF ;

Cheng, N ;

Zlotnick, A ;

Wingfield, PT ;

Stahl, SJ ;

Steven, AC .

NATURE, 1997, 386 (6620) :91-94

← 1 2 3 4 5 →