Interaction of trypan blue with protein and application

The microphase adsorption - spectral correction (MPASC) technique has been described and applied to the aggregation of trypan blue (TB) in proteins. The formation of the microelectrostatic field in protein causes the Langmuir monolayer aggregation of TB. The adsorption ratio of TB to bovine serum albumin (BSA), ovalbumin (OVA), hemoglobin (Hb) and human gamma-globulin (gamma-G) was determined to be 14.8, 8.4, 2.8 and 27.6, respectively, and the adsorption constant of the aggregates to be 7.17 x 10(5), 4.88 x 10(6), 4.85 x 10(6) and 2.99 x 10(6). The adsorption ratio of TB to proteins interestingly indicates almost no relation to the array sequence of amino acid residues. The interaction of TB with proteins is sensitive at pH 3.29, and the reaction was applied to the determination of protein trace with satisfactory results.