Brominated lipids identify lipid binding sites on the surface of the reaction center from Rhodobacter sphaeroides

被引：17

作者：

Roszak, Aleksander W.

Gardiner, Alastair T.

Isaacs, Neil W.

Cogdell, Richard J.

机构：

[1] Univ Glasgow, WestCHEM, Dept Chem, Glasgow G12 8TA, Lanark, Scotland

[2] Univ Glasgow, Glasgow Biomed Res Ctr, IBLS, Div Biochem & Mol Biol, Glasgow G12 8TA, Lanark, Scotland

来源：

BIOCHEMISTRY | 2007年 / 46卷 / 11期

关键词：

D O I：

10.1021/bi062154i

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

This study describes the use of brominated phospholipids to distinguish between lipid and detergent binding sites on the surface of a typical alpha-helical membrane protein. Reaction centers isolated from Rhodobacter sphaeroides were cocrystallized with added brominated phospholipids. X-ray structural analysis of these crystals has revealed the presence of two lipid binding sites from the characteristic strong X-ray scattering from the bromine atoms. These results demonstrate the usefulness of this approach to mapping lipid binding sites at the surface of membrane proteins.

引用

收藏

页码：2909 / 2916

页数：8

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