Identification of human dehydrodolichyl diphosphate synthase gene

被引：46

作者：

Endo, S ^{[1
]}

Zhang, YW ^{[1
]}

Takahashi, S ^{[1
]}

Koyama, T ^{[1
]}

机构：

[1] Tohoku Univ, IMRAM, Aoba Ku, Sendai, Miyagi 9808577, Japan

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 2003年 / 1625卷 / 03期

基金：

日本学术振兴会;

关键词：

dolichol biosynthesis; glycosyl carrier lipid; dehydrodolichyl diphosphate synthase; cis-prenyl chain elongation;

D O I：

10.1016/S0167-4781(02)00628-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We isolated a cDNA encoding human dehydrodolichyl diphosphate (Dedol-PP) synthase and expressed the gene in a yeast mutant strain SNH23-7D, which is deficient in Dedol-PP synthase activity. The identity of the cloned enzyme was confirmed by functional complementation of SNH23-7D strain together with in vitro Dedol-PP synthase activity assay. Northern blot analysis indicated that testis and kidney expressed Dedol-PP synthase mRNA at high levels. (C) 2003 Elsevier Science B.V. All rights reserved.

引用

页码：291 / 295

页数：5

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