Molecular mass estimation by PFG NMR spectroscopy

被引：50

作者：

Crutchfield, Christopher A. ^{[1
]}

Harris, Douglas J. ^{[1
]}

机构：

[1] Cytec Ind Inc, Res & Dev, Stamford, CT 06904 USA

来源：

JOURNAL OF MAGNETIC RESONANCE | 2007年 / 185卷 / 01期

关键词：

molecular mass; diffusion; DOSY; relative diffusivity; PFG NMR;

D O I：

10.1016/j.jmr.2006.12.004

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A simplified PFG NMR diffusion analysis method was developed to estimate the molecular mass of small molecules in dilute aqueous and organic solutions. Internal referencing was utilized to improve the experimental robustness and simplify the data analysis. Specifically, tetramethylsilane (TMS) and HDO were chosen for the organic and aqueous reference molecules, respectively. Relative diffusivity-molecular mass correlations were empirically developed in the range of 2-1280 g/mol for dilute CDCl3 and D2O solutions. The median error in the predicted molecular mass was found to be 10 rel%. The utility of the method was demonstrated by analyzing the major and minor components in olive oil. (c) 2006 Elsevier Inc. All rights reserved.

引用

页码：179 / 182

页数：4

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