Asef, a link between the tumor suppressor APC and G-protein signaling

被引：285

作者：

Kawasaki, Y

Senda, T

Ishidate, T

Koyama, R

Morishita, T

Iwayama, Y

Higuchi, O

Akiyama, T

机构：

[1] Univ Tokyo, Inst Mol & Cellular Biosci, Lab Mol & Genet Informat, Bunkyo Ku, Tokyo 113, Japan

[2] Nagoya Univ, Sch Med, Dept Anat 1, Showa Ku, Nagoya, Aichi 466, Japan

[3] Osaka Univ, Inst Microbial Dis, Dept Oncogene Res, Suita, Osaka 565, Japan

来源：

SCIENCE | 2000年 / 289卷 / 5482期

关键词：

D O I：

10.1126/science.289.5482.1194

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The adenomatous polyposis coli gene (APC) is mutated in familiar adenomatous polyposis and in sporadic colorectal tumors. Here the APC gene product is shown to bind through its armadillo repeat domain to a Rac-specific guanine nucleotide exchange factor (GEF), termed Asef. Endogenous APC colocalized with Asef in mouse colon epithelial cells and neuronal cells. Furthermore, APC enhanced the CEF activity of Asef and stimulated Asef-mediated cell flattening, membrane ruffling, and Lamellipodia formation in MDCK cells. These results suggest that the APC-Asef complex may regulate the actin cytoskeletal network, cell morphology and migration, and neuronal function.

引用

页码：1194 / 1197

页数：6

共 30 条

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