Characterization of diacetylenic liposomes as carriers for oral vaccines

被引：38

作者：

Alonso-Romanowski, S ^{[1
]}

Chiaramoni, NS ^{[1
]}

Lioy, VS ^{[1
]}

Gargini, RA ^{[1
]}

Viera, LI ^{[1
]}

Taira, MC ^{[1
]}

机构：

[1] Univ Nacl Quilmes, Dept Sci & Technol, Lab Biomembranes, RA-1876 Bernal, Argentina

来源：

CHEMISTRY AND PHYSICS OF LIPIDS | 2003年 / 122卷 / 1-2期

关键词：

EVs; diacetylenic lipids; polymerization; oral delivery; stability digestive tract;

D O I：

10.1016/S0009-3084(02)00190-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In order to evaluate liposomes as vehicle for oral vaccines the characterization and stability of polymerized and non-polymerized liposomes were examined. Mixtures of 1,2-bis(10,12-tricosadiynoyl)-sn-glycero-3 phosphocholine) (DC8,9PC) with saturated 1,2-dimiristoyl-sn-glycero-3-phosphocholine in molar ratio 1:1 were used. Saturated and non-saturated lipids were combined to give a chemically modified membrane by UV polymerization derived from DC8,9PC. Characterization was carried out by electronic microscopy, differential scanning calorimetry (DSC) and by hydrophobicity factor (HF). The stability towards the digestive tract (including saliva): acidic solutions, bile and pancreatin are compared to buffer pH 7.4, measuring the release of Glucose-6-phosphate or bovine plasma albumin entrapment. The polymerized liposomes showed further augmentation of the HF and the size. DSC showed phase separation and lower Tt if compared to data obtained for DC8,9PC. The HF, as main factor is discussed in relation to in vitro stability, suggesting that polymerized and non-polymerized liposomes would serve effectively as an oral delivery vehicle. (C) 2002 Elsevier Science Ireland Ltd. All rights reserved.

引用

页码：191 / 203

页数：13

共 29 条

[21] STRUCTURE OF POLYMERIZABLE LIPID BILAYERS .4. MIXTURES OF LONG-CHAIN DIACETYLENIC AND SHORT CHAIN SATURATED PHOSPHATIDYLCHOLINES AND ANALOGOUS ASYMMETRIC ISOMERS [J].

RHODES, DG ;

SINGH, A .

CHEMISTRY AND PHYSICS OF LIPIDS, 1991, 59 (03) :215-224

[22]

SINGH A, 1988, APPL BIOACTIVE POLYM, P239

[23] MEASUREMENT OF PROTEIN USING BICINCHONINIC ACID [J].

SMITH, PK ;

KROHN, RI ;

HERMANSON, GT ;

MALLIA, AK ;

GARTNER, FH ;

PROVENZANO, MD ;

FUJIMOTO, EK ;

GOEKE, NM ;

OLSON, BJ ;

KLENK, DC .

ANALYTICAL BIOCHEMISTRY, 1985, 150 (01) :76-85

[24] Controlling the morphology of chiral lipid tubules [J].

Spector, MS ;

Selinger, JV ;

Singh, A ;

Rodriguez, JM ;

Price, RR ;

Schnur, JM .

LANGMUIR, 1998, 14 (13) :3493-3500

[25] Formation of polymerizable phospholipid nanotubules and their transformation into a network gel [J].

Svenson, S ;

Messersmith, PB .

LANGMUIR, 1999, 15 (13) :4464-4471

[26]

Viera L, 1998, BIOPHYS J, V74, pA12

[27]

WEBER K, 1969, J BIOL CHEM, V244, P4406

[28]

WORTHINGTON CC, 1988, GLUCOSE 6 PHOSPHATE, P159

[29] THE MECHANISM OF FORMATION OF LIPID TUBULES FROM LIPOSOMES [J].

YAGER, P ;

PRICE, RR ;

SCHNUR, JM ;

SCHOEN, PE ;

SINGH, A ;

RHODES, DG .

CHEMISTRY AND PHYSICS OF LIPIDS, 1988, 46 (03) :171-179

← 1 2 3 →