Fluorescent Conjugated Polyfluorene with Pendant Lactopyranosyl Ligands for Studies of Ca2+-Mediated Carbohydrate-Carbohydrate Interaction

被引：36

作者：

Chen, Qi ^{[2
]}

Cui, Yi ^{[1
]}

Zhang, Tian-Long ^{[2
]}

Cao, Jie ^{[1
]}

Han, Bao-Hang ^{[2
]}

机构：

[1] Chinese Acad Sci, Grad Univ, Beijing 100049, Peoples R China

[2] Natl Ctr Nanosci & Technol, Beijing 100190, Peoples R China

来源：

BIOMACROMOLECULES | 2010年 / 11卷 / 01期

基金：

美国国家科学基金会;

关键词：

SURFACE-PLASMON RESONANCE; FLUORENE-BASED GLYCOPOLYMERS; GOLD GLYCONANOPARTICLES; ESCHERICHIA-COLI; SELF-RECOGNITION; CLICK CHEMISTRY; POLYMERS; MODEL; LECTINS; CELLS;

D O I：

10.1021/bm901165n

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A well-defined fluorescent conjugated polyfluorene with pendant lactopyranosyl ligands was easily prepared through Cu(I)-catalyzed azide/alkyne "click" ligation and Suzuki coupling polymerization. As a fluorescent multivalent model system of glycoconjugates, the polymer was first used for studies of metal ion-mediated carbohydrate-carbohydrate interaction based on fluorescence spectroscopy. A significant fluorescence quenching of the lactosyl-bearing polyfluorene was observed upon addition of calcium ion, which is attributed to the polymer aggregation derived from Ca2+-mediated complex formation. Dynamic light scattering can also prove Ca2+-induced aggregation of the polymer based on determination of the corresponding hydrodynamic diameters. The calcium-mediated lactose-lactose interaction was reversible when treated with EDTA. In control studies, Ca2+-induced fluorescence quenching can not be observed for cellobiosyl- or galactosyl-functionalized polymer analogues, which show that specific sugar structures are critical for carbohydrate-metal complex formation.

引用

页码：13 / 19

页数：7

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