Detection of interleukin-2 in addition to interferon-γ discriminates active tuberculosis patients, latently infected individuals, and controls

被引：102

作者：

Biselli, R. ^{[2
]}

Mariotti, S.

Sargentini, V.

Sauzullo, I. ^{[3
]}

Lastilla, M. ^{[2
]}

Mengoni, F. ^{[3
]}

Vanini, V. ^{[4
]}

Girardi, E. ^{[4
]}

Goletti, D. ^{[4
]}

D' Amelio, R. ^{[3
,5
]}

Nisini, R. ^{[1
]}

机构：

[1] Ist Super Sanita, Dipartimento Malattie Infett Parassitarie & Immun, I-00161 Rome, Italy

[2] Serv Sanit, Rome, Italy

[3] Sapienza Univ Roma, Rome, Italy

[4] Ist Nazl Malattie Infett L Spallanzani, Rome, Italy

[5] Minist Difesa, Direz Gen Sanita Mil, Rome, Italy

来源：

CLINICAL MICROBIOLOGY AND INFECTION | 2010年 / 16卷 / 08期

关键词：

Diagnosis; IFN-gamma; IL-2; latent infection; tuberculosis; T-CELLS; IFN-GAMMA; DIAGNOSIS; ASSAYS;

D O I：

10.1111/j.1469-0691.2009.03104.x

中图分类号：

R51 [传染病];

学科分类号：

100201 [内科学];

摘要：

P>Effective control of tuberculosis (TB) includes discrimination of subjects with active TB from individuals with latent TB infection (LTBI). As distinct interferon (IFN)-gamma and interleukin (IL)-2 profiles of antigen-specific T-cells have been associated with different clinical stages and antigen loads in several viral and bacterial diseases, we analysed these cytokines in TB using a modified QuantiFERON-TB Gold In Tube test. Detection of IL-2 in addition to IFN-gamma distinguishes not only Mycobacterium tuberculosis-infected subjects from healthy controls, but also individuals with LTBI from active TB patients. This may help to improve diagnostic tests for TB.

引用

页码：1282 / 1284

页数：3

共 12 条

[1]

Mapping immune reactivity toward Rv2653 and Rv2654:: Two novel low-molecular-mass antigens found specifically in the Mycobacterium tuberculosis complex [J].

Aagaard, C ;

Brock, I ;

Olsen, A ;

Ottenhoff, THM ;

Weldingh, K ;

Andersen, P .

JOURNAL OF INFECTIOUS DISEASES, 2004, 189 (05) :812-819

[2]

Accuracy of an immune diagnostic assay based on RD1 selected epitopes for active tuberculosis in a clinical setting:: a pilot study [J].

Goletti, D. ;

Carrara, S. ;

Vincenti, D. ;

Saltini, C. ;

Rizzi, E. Busi ;

Schinina, V. ;

Ippolito, G. ;

Amicosante, M. ;

Girardi, E. .

CLINICAL MICROBIOLOGY AND INFECTION, 2006, 12 (06) :544-550

[3]

Region of difference 1 antigen-specific CD4+ memory T cells correlate with a favorable outcome of tuberculosis [J].

Goletti, Delia ;

Butera, Ornella ;

Bizzoni, Federica ;

Casetti, Rita ;

Girardi, Enrico ;

Poccia, Fabrizio .

JOURNAL OF INFECTIOUS DISEASES, 2006, 194 (07) :984-992

[4]

THE TUBERCULIN SKIN-TEST [J].

HUEBNER, RE ;

SCHEIN, MF ;

BASS, JB .

CLINICAL INFECTIOUS DISEASES, 1993, 17 (06) :968-975

[5]

Dynamics of T lymphocyte responses: Intermediates, effectors, and memory cells [J].

Lanzavecchia, A ;

Sallusto, F .

SCIENCE, 2000, 290 (5489) :92-97

[6]

Dynamic relationship between IFN-γ and IL-2 profile of Mycobacterium tuberculosis-specific T cells and antigen load [J].

Millington, Kerry A. ;

Innes, John A. ;

Hackforth, Sarah ;

Hinks, Timothy S. C. ;

Deeks, Jonathan J. ;

Dosanjh, Davinder P. S. ;

Guyot-Revol, Valerie ;

Gunatheesan, Rubamalaar ;

Klenerman, Paul ;

Lalvani, Ajit .

JOURNAL OF IMMUNOLOGY, 2007, 178 (08) :5217-5226

[7]

Interferonn assays-γ in the immunodiagnosis of tuberculosis:: a systematic review [J].

Pai, M ;

Riley, LW ;

Colford, JM .

LANCET INFECTIOUS DISEASES, 2004, 4 (12) :761-776

[8]

Direct ex vivo analysis of antigen-specific IFN-γ-secreting CD4 T cells in Mycobacterium tuberculosis-infected individuals:: Associations with clinical disease state and effect of treatment [J].

Pathan, AA ;

Wilkinson, KA ;

Klenerman, P ;

McShane, H ;

Davidson, RN ;

Pasvol, G ;

Hill, AVS ;

Lalvani, A .

JOURNAL OF IMMUNOLOGY, 2001, 167 (09) :5217-5225

[9]

Improving T-Cell Assays for the Diagnosis of Latent TB Infection: Potential of a Diagnostic Test Based on IP-10 [J].

Ruhwald, Morten ;

Petersen, Janne ;

Kofoed, Kristian ;

Nakaoka, Hiroshi ;

Cuevas, Luis Eduardo ;

Lawson, Lovett ;

Squire, Stephen Bertil ;

Eugen-Olsen, Jesper ;

Ravn, Pernille .

PLOS ONE, 2008, 3 (08)

[10]

Cytometric detection of antigen-specific IFN-γ/IL-2 secreting cells in the diagnosis of tuberculosis [J].

Sargentini, Valeria ;

Mariotti, Sabrina ;

Carrara, Stefania ;

Gagliardi, Maria Cristina ;

Teloni, Raffaela ;

Goletti, Delia ;

Nisini, Roberto .

BMC INFECTIOUS DISEASES, 2009, 9

← 1 2 →