Gene expression profiling:: monitoring transcription and translation products using DNA microarrays and proteomics

被引：228

作者：

Celis, JE

Kruhoffer, M

Gromova, I

Frederiksen, C

Ostergaard, M

Thykjaer, T

Gromov, P

Yu, JS

Pálsdóttir, H

Magnusson, N

Orntoft, TF

机构：

[1] Aarhus Univ, Dept Med Biochem, DK-8000 Aarhus C, Denmark

[2] Aarhus Univ, Danish Ctr Human Genome Res, DK-8000 Aarhus C, Denmark

[3] Aarhus Univ Hosp, Dept Clin Biochem, Mol Diagnost Lab, DK-8200 Aarhus N, Denmark

来源：

FEBS LETTERS | 2000年 / 480卷 / 01期

关键词：

gene expression profile; microarray; proteome; two-dimensional gel electrophoresis; immunohistochemistry; bladder cancer;

D O I：

10.1016/S0014-5793(00)01771-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens. (C) 2000 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：2 / 16

页数：15

共 155 条

[21]

Celis A, 1999, ELECTROPHORESIS, V20, P355, DOI 10.1002/(SICI)1522-2683(19990201)20:2<355::AID-ELPS355>3.0.CO

[22]

2-N

[23]

Celis J. E, 1996, ELECTROPHORESIS, V17, P1653

[24]

Celis JE, 1999, CANCER RES, V59, P3003

[25]

Celis JE, 1996, CANCER RES, V56, P4782

[26]

Celis JE, 1999, ELECTROPHORESIS, V20, P300

[27] Human and mouse proteomic databases: novel resources in the protein universe [J].

Celis, JE ;

Ostergaard, M ;

Jensen, NA ;

Gromova, I ;

Rasmussen, HH ;

Gromov, P .

FEBS LETTERS, 1998, 430 (1-2) :64-72

[28] The human keratinocyte two-dimensional gel protein database (update 1995): Mapping components of signal transduction pathways [J].

Celis, JE ;

Rasmussen, HH ;

Gromov, P ;

Olsen, E ;

Madsen, P ;

Leffers, H ;

Honore, B ;

Dejgaard, K ;

Vorum, H ;

Kristensen, DB ;

Ostergaard, M ;

Haunso, A ;

Jensen, NA ;

Celis, A ;

Basse, B ;

Lauridsen, JB ;

Ratz, GP ;

Andersen, AH ;

Walbum, E ;

Kjaergaard, I ;

Andersen, I ;

Puype, M ;

VanDamme, J ;

Vandekerckhove, J .

ELECTROPHORESIS, 1995, 16 (12) :2177-+

[29] Bladder squamous cell carcinomas express psoriasin and externalize it to the urine [J].

Celis, JE ;

Rasmussen, HH ;

Vorum, H ;

Madsen, P ;

Honore, B ;

Wolf, H ;

Orntoft, TF .

JOURNAL OF UROLOGY, 1996, 155 (06) :2105-2112

[30] 2D protein electrophoresis: can it be perfected? [J].

Celis, JE ;

Gromov, P .

CURRENT OPINION IN BIOTECHNOLOGY, 1999, 10 (01) :16-21

← 1 2 3 4 5 6 7 8 9 10 →