The measurement of protein synthesis for assessing proteostasis in studies of slowed aging

被引：38

作者：

Miller, Benjamin F. ^{[1
]}

Drake, Joshua C. ^{[1
]}

Naylor, Bradley ^{[2
]}

Price, John C. ^{[2
]}

Hamilton, Karyn L. ^{[1
]}

机构：

[1] Colorado State Univ, Dept Hlth & Exercise Sci, Ft Collins, CO 80523 USA

[2] Brigham Young Univ, Dept Chem & Biochem, Provo, UT 84601 USA

来源：

AGEING RESEARCH REVIEWS | 2014年 / 18卷

关键词：

Stable isotope; Deuterium oxide; Long-lived model; Mitochondria; Proliferation; EXTENDS LIFE-SPAN; SKELETAL-MUSCLE; MITOCHONDRIAL BIOGENESIS; CALORIE RESTRICTION; FOOD RESTRICTION; CELL-SIZE; IN-VIVO; MICE; ACTIVATION; EXERCISE;

D O I：

10.1016/j.arr.2014.09.005

中图分类号：

Q2 [细胞生物学];

学科分类号：

071013 [干细胞生物学];

摘要：

Slowing the aging process can reduce the risk for multiple chronic diseases simultaneously. It is increasingly recognized that maintaining protein homeostasis (or proteostasis) is important for slowing the aging process. Since proteostasis is a dynamic process, monitoring it is not a simple task and requires use of appropriate methods. This review will introduce methods to assess protein and DNA synthesis using deuterium oxide (D2O), and how protein and DNA synthesis outcomes provide insight into proteostatic mechanisms. Finally, we provide a discussion on how these assessments of protein and DNA synthesis are "mechanistic" investigations and provide an appropriate framework for the further development of slowed aging treatments. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：106 / 111

页数：6

共 46 条

[1]

Translational control mechanisms in metabolic regulation: critical role of RNA binding proteins, microRNAs, and cytoplasmic RNA granules [J].