Structure of limonene synthase, a simple model for terpenoid cyclase catalysis

被引：179

作者：

Hyatt, David C.

Youn, Buhyun

Zhao, Yuxin

Santhamma, Bindu

Coates, Robert M.

Croteau, Rodney B. ^{[1
]}

Kang, Chulhee

机构：

[1] Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA

[2] Washington State Univ, Sch Mol Biosci, Pullman, WA 99164 USA

[3] Univ Illinois, Dept Chem, Urbana, IL 61801 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2007年 / 104卷 / 13期

关键词：

crystal structure; geranyl diphosphate; linalyl diphosphate; monoterpene cyclase; monoterpene synthase;

D O I：

10.1073/pnas.0700915104

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The crystal structure of (4S)-limonene synthase from Meniha spicata, a metal ion-dependent monoterpene cyclase that catalyzes the coupled isomerization and cyclization of geranyl diphosphate, is reported at 2.7-angstrom resolution in two forms liganded to the substrate and intermediate analogs, 2-fluorogeranyl diphosphate and 2-fluorolinalyl diphosphate, respectively. The implications of these findings are described for domain interactions in the homodimer and for changes in diphosphate-metal ion coordination and substrate binding conformation in the course of the multistep reaction.

引用

页码：5360 / 5365

页数：6

共 61 条

[41] Bifunctional abietadiene synthase: Free diffusive transfer of the (+)-copalyl diphosphate intermediate between two distinct active sites [J].