Antisense silencing of the creA gene in Aspergillus nidulans

被引：23

作者：

Bautista, LF ^{[1
]}

Aleksenko, A ^{[1
]}

Hentzer, M ^{[1
]}

Santerre-Henriksen, A ^{[1
]}

Nielsen, J ^{[1
]}

机构：

[1] Tech Univ Denmark, Dept Biotechnol, Ctr Proc Biotechnol, DK-2800 Lyngby, Denmark

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2000年 / 66卷 / 10期

关键词：

D O I：

10.1128/AEM.66.10.4579-4581.2000

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Antisense expression of a portion of the gene encoding the major carbon catabolite repressor CREA in Aspergillus nidulans resulted in a substantial increase in the levels of glucose-repressible enzymes, both endogenous and heterologous, in the presence of glucose. The derepression effect was approximately one-half of that achieved in a null creA mutant. Unlike results for that mutant, however, growth parameters and colony morphology in the antisense transformants were not affected.

引用

页码：4579 / 4581

页数：3

共 17 条

[11]

Kitamoto N, 1999, APPL ENVIRON MICROB, V65, P20

[12] INDUCTION AND REPRESSION OF ALPHA-AMYLASE PRODUCTION IN BATCH AND CONTINUOUS CULTURES OF ASPERGILLUS-ORYZAE [J].

MORKEBERG, R ;

CARLSEN, M ;

NIELSEN, J .

MICROBIOLOGY-SGM, 1995, 141 :2449-2454

[13]

MULANEY EJ, 1985, MOL GEN GENET, V199, P37

[14] WHAT MAKES AN MESSENGER-RNA ANTI-SENSE-ITIVE [J].

NELLEN, W ;

LICHTENSTEIN, C .

TRENDS IN BIOCHEMICAL SCIENCES, 1993, 18 (11) :419-423

[15]

Sambrook J., 2002, MOL CLONING LAB MANU

[16] Null alleles of creA, the regulator of carbon catabolite repression in Aspergillus nidulans [J].

Shroff, RA ;

OConnor, SM ;

Hynes, MJ ;

Lockington, RA ;

Kelly, JM .

FUNGAL GENETICS AND BIOLOGY, 1997, 22 (01) :28-38

[17] Construction of a low-serine-type-carboxypeptidase-product mutant of Aspergillus oryzae by the expression of antisense RNA and its use as a host for heterologous protein secretion [J].

Zheng, XF ;

Kobayashi, Y ;

Takeuchi, M .

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 1998, 49 (01) :39-44

← 1 2 →