Site-specific incorporation of the 1-hexanol-1,N6-etheno-2′-deoxyadenosine adduct into oligodeoxyribonucleotides

被引：5

作者：

Carvalho, VM

Gasparutto, D

Di Mascio, P

Medeiros, MHG

Cadet, J ^{[1
]}

机构：

[1] CEA, SCIB, DRFMC, FRE 2600,Lab Les Acides Nucl, F-38054 Grenoble 9, France

[2] Univ Sao Paulo, Inst Quim, Dept Bioquim, BR-05513970 Sao Paulo, Brazil

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2003年 / 11卷 / 11期

基金：

巴西圣保罗研究基金会;

关键词：

D O I：

10.1016/S0968-0896(03)00125-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Modified oligonucleotides that contain the hydrophobic 1-hexanol-1,N-6-etheno-2'-deoxyadenosine adduct have been synthesized using a mild solid phase phosphoramidite chemistry. The presence and the integrity of the modified nucleoside in the synthetic oligomers were confirmed by electrospray ionization and MALDI mass spectrometry measurements together with analysis of the complete enzymatic hydrolysate by high performance liquid chromatography coupled to UV and fluorescent detection techniques. (C) 2003 Elsevier Science Ltd. All rights reserved.

引用

页码：2445 / 2452

页数：8

共 23 条

[1] Etheno-adduct-forming chemicals: from mutagenicity testing to tumor mutation spectra [J].