Cloning and characterization of the mouse pituitary adenylate cyclase-activating polypeptide (PACAP) gene

被引：59

作者：

Yamamoto, K ^{[1
]}

Hashimoto, H ^{[1
]}

Hagihara, N ^{[1
]}

Nishino, A ^{[1
]}

Fujita, T ^{[1
]}

Matsuda, T ^{[1
]}

Baba, A ^{[1
]}

机构：

[1] Osaka Univ, Fac Pharmaceut Sci, Dept Pharmacol, Suita, Osaka 5650871, Japan

来源：

GENE | 1998年 / 211卷 / 01期

关键词：

VIP; genomic organization; 5 '-flanking region; promoter activity; 5 '-RACE; alternative splicing;

D O I：

10.1016/S0378-1119(98)00110-3

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The gene encoding the mouse precursor of pituitary adenylate cyclase-activating polypeptide (PACAP) has been cloned, and its structural organization was determined. Using the 5'-rapid amplification of cDNA ends (RACE) procedure, three types of transcription initiation produced by alternative exon usage of two untranslated alternative exons (exons 1A and 1B) were defined. The PA CAP gene spans 6.6 kb of genomic DNA and is composed of six exons including the alternative exons. The signal peptide, PACAP-related peptide and mature 38-amino acid PACAP (PACAP-38) are encoded within exons 2, 4 and 5, respectively. The 5'-flanking region of the PACAP gene contains several sequence motifs homologous to cAMP response element, TPA response element, and growth hormone factor-1 binding site. A dinucleotide repeat sequence is present in an intron. In addition, there are di-and tetranucleotide repeat sequences 2.4 kb and 3.2 kb upstream to the translation start point, respectively. The overall intron-exon organization and the production of the alternate mRNAs of the PA CAP gene are markedly similar to those of the growth hormone-releasing hormone (GHRH), supporting the hypothesis that the two genes encoding GHRH or PACAP were originated from a gene duplication. Promoter analysis of the 5'-flanking region of the PACAP gene using a luciferase gene reporter system revealed that the isolated 5'-flanking region has functional promoter activity and is responsible for inducible expression. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：63 / 69

页数：7

共 25 条

[1] STRUCTURE OF THE GENE ENCODING THE MOUSE PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE RECEPTOR [J].

AINO, H ;

HASHIMOTO, H ;

OGAWA, N ;

NISHINO, A ;

YAMAMOTO, K ;

NOGI, H ;

NAGATA, S ;

BABA, A .

GENE, 1995, 164 (02) :301-304

[2] PHORBOL ESTER INDUCIBLE GENES CONTAIN A COMMON CIS ELEMENT RECOGNIZED BY A TPA-MODULATED TRANS-ACTING FACTOR [J].

ANGEL, P ;

IMAGAWA, M ;

CHIU, R ;

STEIN, B ;

IMBRA, RJ ;

RAHMSDORF, HJ ;

JONAT, C ;

HERRLICH, P ;

KARIN, M .

CELL, 1987, 49 (06) :729-739

[3]

ARIMURA A, 1992, REGUL PEPTIDES, V37, P287

[4] THE PITUITARY-SPECIFIC TRANSCRIPTION FACTOR-GHF-1 IS A HOMEOBOX-CONTAINING PROTEIN [J].

BODNER, M ;

CASTRILLO, JL ;

THEILL, LE ;

DEERINCK, T ;

ELLISMAN, M ;

KARIN, M .

CELL, 1988, 55 (03) :505-518

[5]

BREATHNACH R, 1981, ANNU REV BIOCHEM, V50, P349, DOI 10.1146/annurev.bi.50.070181.002025

[6] TYPE-I RECEPTORS FOR PACAP (A NEUROPEPTIDE EVEN MORE IMPORTANT THAN VIP) [J].

CHRISTOPHE, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1154 (02) :183-199

[7] CYCLIC-AMP AND PHORBOL ESTER-STIMULATED TRANSCRIPTION MEDIATED BY SIMILAR DNA ELEMENTS THAT BIND DISTINCT PROTEINS [J].

DEUTSCH, PJ ;

HOEFFLER, JP ;

JAMESON, JL ;

HABENER, JF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7922-7926

[8] RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].

FROHMAN, MA ;

DUSH, MK ;

MARTIN, GR .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002

[9] MOLECULAR-CLONING AND TISSUE DISTRIBUTION OF A RECEPTOR FOR PITUITARY ADENYLATE CYCLASE-ACTIVATING POLYPEPTIDE [J].

HASHIMOTO, H ;

ISHIHARA, T ;

SHIGEMOTO, R ;

MORI, K ;

NAGATA, S .

NEURON, 1993, 11 (02) :333-342

[10]

Hashimoto H, 1996, J COMP NEUROL, V371, P567

← 1 2 3 →