Molecular tripods showing fluorescence enhancement upon binding to streptavidin

被引：16

作者：

Kim, TW ^{[1
]}

Yoon, HY

Park, JH

Kwon, OH

Jang, DJ

Hong, JI

机构：

[1] Seoul Natl Univ, Coll Nat Sci, Dept Chem, Seoul 151747, South Korea

[2] Korea Adv Inst Sci & Technol, Ctr Mol Design & Synth, Taejon 305701, South Korea

来源：

ORGANIC LETTERS | 2005年 / 7卷 / 01期

关键词：

D O I：

10.1021/ol047801h

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

We introduce a new approach to biotin-streptavidin assays based on a molecular tripod which consists of biotin, a fluorophore, and a quencher. The interaction between streptavidin and molecular tripods perturbs the ground-state quencher-fluorophore dimeric conformation in the absence of streptavidin and diminishes the intrinsic self-quenching of a quencher-fluorophore pair. The emission intensity of the molecular tripods plus streptavidin is 3.5-5.2 times that of molecular tripods in the absence of streptavidin.

引用

页码：111 / 114

页数：4