New scheme of the biosynthesis of formononetin involving 2,7,4′-trihydroxyisofiavanone but not daidzein as the methyl acceptor

被引:20
作者
Akashi, T [1 ]
Sawada, Y [1 ]
Aoki, T [1 ]
Ayabe, S [1 ]
机构
[1] Nihon Univ, Dept Appl Biol Sci, Fujisawa, Kanagawa 2528510, Japan
关键词
Glycyrrhiza echinata; Fabaceae; cytochrome p450; flavonoid; O-methyltransferase;
D O I
10.1271/bbb.64.2276
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Glycyrrhiza echinata cell-free extract produced isoformononetin by the 7-O-transmethylation of daidzein from S-adenosyl-L-methionine (SAM), When the yeast microsome expressing 2-hydroxyisoflavanone synthase was mixed with the cell-free extract and incubated with liquiritigenin and SAM, formononetin emerged, Furthermore, the cell-free extract yielded formononetin on incubation with 2,7,4'-trihydroxyisoflavanone and SAM. We propose a novel pathway of formononetin biosynthesis involving 2,7,4'-trihydroxyisoflavanone as the methyl acceptor.
引用
收藏
页码:2276 / 2279
页数:4
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