Spectrophotometric method for determination and kinetics of amino acids through their reaction with syringaldehyde

被引：16

作者：

Medien, HAA ^{[1
]}

机构：

[1] Ain Shams Univ, Fac Sci, Dept Chem, Cairo, Egypt

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 1998年 / 54卷 / 02期

关键词：

spectrophotometry; amino acids; syringaldehyde;

D O I：

10.1016/S1386-1425(98)00004-3

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

A spectrophotometric method is described for the determination of amino acids. The method is based on the reaction between amino acids and syringaldehyde at pH 9.0, by which a color is developed with maximum absorption at 420 nm in aqueous methyl alcohol. The absorption of the product obeys Beer's law within the concentration range of 0.025-0.5 mM of original amino acid. The kinetics of the reaction follows overall second order kinetics, first order in each of the reactants. The rates of the reaction were investigated as a function of pH of the reaction medium and structure of the amino compounds. Logarithms of the second-order rate constants increased with amino acid anion concentration as the pH was increased. The mechanisms of the reaction have been discussed. (C) 1998 Elsevier Science B.V.

引用

页码：359 / 365

页数：7

共 36 条

[1] IRON(III) TYROSINATE MODELS - SYNTHESIS AND SPECTROSCOPIC AND STEREOCHEMICAL STUDIES OF IRON(III) COMPLEXES OF N-SALICYLIDENE-L-AMINO ACIDS [J].