Specific Recognition of Influenza A/H1N1/2009 Antibodies in Human Serum: A Simple Virus-Free ELISA Method

被引:32
作者
Alvarez, Mario M. [1 ]
Lopez-Pacheco, Felipe [1 ]
Aguilar-Yanez, Jose M. [1 ]
Portillo-Lara, Roberto [1 ]
Mendoza-Ochoa, Gonzalo I. [1 ]
Garcia-Echauri, Sergio [1 ]
Freiden, Pamela [2 ]
Schultz-Cherry, Stacey [2 ]
Zertuche-Guerra, Manuel I. [1 ]
Bulnes-Abundis, David [1 ]
Salgado-Gallegos, Johari [1 ]
Elizondo-Montemayor, Leticia [1 ]
Hernandez-Torre, Martin [1 ]
机构
[1] Tecnol Monterrey, Ctr Biotecnol FEMSA, Monterrey, New Leon, Mexico
[2] St Jude Childrens Res Hosp, Dept Infect Dis, Memphis, TN 38105 USA
来源
PLOS ONE | 2010年 / 5卷 / 04期
基金
美国国家卫生研究院;
关键词
A H5N1 VIRUS; HEMAGGLUTINATION INHIBITION; ANTIGENIC STRUCTURE; RECEPTOR-BINDING; CELL-CULTURE; VACCINE; BURDEN; STRAIN; SITES; ASSAY;
D O I
10.1371/journal.pone.0010176
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Background: Although it has been estimated that pandemic Influenza A H1N1/2009 has infected millions of people from April to October 2009, a more precise figure requires a worldwide large-scale diagnosis of the presence of Influenza A/H1N1/2009 antibodies within the population. Assays typically used to estimate antibody titers (hemagglutination inhibition and microneutralization) would require the use of the virus, which would seriously limit broad implementation. Methodology/Principal Findings: An ELISA method to evaluate the presence and relative concentration of specific Influenza A/H1N1/2009 antibodies in human serum samples is presented. The method is based on the use of a histidine-tagged recombinant fragment of the globular region of the hemagglutinin (HA) of the Influenza A H1N1/2009 virus expressed in E. coli. Conclusions/Significance: The ELISA method consistently discerns between Inf A H1N1 infected and non-infected subjects, particularly after the third week of infection/exposure. Since it does not require the use of viral particles, it can be easily and quickly implemented in any basic laboratory. In addition, in a scenario of insufficient vaccine availability, the use of this ELISA could be useful to determine if a person has some level of specific antibodies against the virus and presumably at least partial protection.
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页数:14
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