Cloning and characterization of a gene cluster for geldanamycin production in Streptomyces hygroscopicus NRRL 3602

被引：137

作者：

Rascher, A

Hu, ZH

Viswanathan, N

Schirmer, A

Reid, R

Nierman, WC

Lewis, M

Hutchinson, CR

机构：

[1] Kosan Biosci, Hayward, CA 94545 USA

[2] Inst Genome Res, Rockville, MD 20850 USA

来源：

FEMS MICROBIOLOGY LETTERS | 2003年 / 218卷 / 02期

关键词：

ansamycin; antitumor; biosynthesis; cancer chemotherapy; Hsp90; polyketide synthase;

D O I：

10.1016/S0378-1097(02)01148-5

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We illustrate the use of a PCR-based method by which the genomic DNA of a microorganism can be rapidly queried for the presence of type I modular polyketide synthase genes to clone and characterize, by sequence analysis and gene disruption, a major portion of the geldanamycin production gene cluster from Streptomyces hygroscopicus var. geldanus NRRL 3602. (C) 2002 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：223 / 230

页数：8

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