Vancomycin covalently bonded to titanium alloy prevents bacterial colonization

被引:120
作者
Antoci, Valentin, Jr.
King, Samuel B.
Jose, Binoy
Parvizi, Javad
Zeiger, Allen R.
Wickstrom, Eric
Freeman, Theresa A.
Cornposto, Russell J.
Ducheyne, Paul
Shapiro, Irving M.
Hickok, Noreen J.
Adams, Christopher S.
机构
[1] Thomas Jefferson Univ, Dept Orthopaed Surg, Philadelphia, PA 19107 USA
[2] SK Biopharmaceut, Fairfield, NJ 07004 USA
[3] Rothman Inst, Philadelphia, PA 19107 USA
[4] Univ Penn, Sch Dent Med, Dept Orthodont, Philadelphia, PA 19104 USA
[5] Thomas Jefferson Univ, Dept Biochem & Mol Biol, Philadelphia, PA 19107 USA
[6] Univ Penn, Dept Mat Sci & Engn, Philadelphia, PA 19104 USA
[7] Univ Penn, Dept Bioengn, Philadelphia, PA 19104 USA
关键词
implant; infection; vancomycin; S; aureus; titanium;
D O I
10.1002/jor.20348
中图分类号
R826.8 [整形外科学]; R782.2 [口腔颌面部整形外科学]; R726.2 [小儿整形外科学]; R62 [整形外科学(修复外科学)];
学科分类号
摘要
Periprosthetic infection is a devastating consequence of implant insertion and can arise from hematogenous sources or surgical contamination. Microbes can preferentially colonize the implant surface and, by forming a biofilm, escape immune surveillance. We hypothesized that if an antibiotic can be tethered to a titanium alloy (Ti) surface, it will inhibit bacterial colonization, prevent biofilm formation, and avert late-stage infection. To test this hypothesis, a Ti rod was covalently derivatized with vancomycin. Reaction efficiencies were evaluated by colorimetric and spectrophotometric measurements. The vancomycin-modified surface was stable in aqueous solutions over extended time periods and maintained antibiotic coverage, even after press-fit insertion into a cadaverous rat femora. When evaluated using fluorescently labeled bacteria, or by direct colony counts, the surface-bound antibiotic prevented bacterial colonization in vitro after: (1) exposure to high levels of S. aureus; (2) extended incubation in physiological buffers; and (3) repeated bacterial challenges. Importantly, whereas the vancomycin-derivitized pins prevented bacterial colonization, S. aureus adhered to control pins, even in the presence of concentrations of vancomycin that exceeded the strain MIC. These results demonstrate that we have effectively engineered a stable, bactericidal Ti surface. This new surface holds great promise in terms of mitigating or preventing periprosthetic infection. (c) 2007 Orthopaedic Research Society. Published by Wiley Periodicals, Inc.
引用
收藏
页码:858 / 866
页数:9
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