Aldehydes in exhaled breath condensate of patients with chronic obstructive pulmonary disease

被引:160
作者
Corradi, M
Rubinstein, I
Andreoli, R
Manini, P
Caglieri, A
Poli, D
Alinovi, R
Mutti, A
机构
[1] Univ Parma, Lab Ind Toxicol, Dept Clin Med Nephrol & Hlth Sci, I-43100 Parma, Italy
[2] Univ Parma, Natl Inst Occupat Safety, Dep Clin Med Nephrol & Hlth Sci, I-43100 Parma, Italy
[3] Univ Parma, Prevent Res Ctr, Dept Clin Med Nephrol & Hlth Sci, I-43100 Parma, Italy
[4] Univ Illinois, Coll Med, Dept Resp & Crit Care Med, Chicago, IL USA
[5] Univ Illinois, Coll Pharm, Dept Pharmaceut & Pharmacodynam, Chicago, IL USA
[6] Vet Affairs Chicago Hlth Care Syst, W Side Div, Chicago, IL USA
关键词
alclehydes; biomarkers; COPD; exhaled breath condensate; reproducibility;
D O I
10.1164/rccm.200210-1253OC
中图分类号
R4 [临床医学];
学科分类号
1002 ; 100602 ;
摘要
The aims of the present study were (1) to evaluate whether individual aldehydes resulting from lipid peroxidation can be measured in exhaled breath condensate, (2) to assess the influence of sampling procedures on aldehyde concentrations, and (3) to compare aldehyde levels of patients with stable, moderate to severe, chronic obstructive pulmonary disease with those of smoking and nonsmoking control subjects. Aldehydes (malondialdehyde, hexanal, heptanal, and nonanal) were measured by liquid chromatography-tandem mass spectrometry in all samples and overlapping results were obtained by different sampling procedures. Malondialdehyde (57.2 +/- 2.4 nmol/L), hexanal (63.5 +/- 4.4 nmol/L), and heptanal (26.6 +/- 3.9 nmol/L) were increased in patients as compared with nonsmoking control subjects (17.7 +/- 5.5 nmol/L, p < 0.0001; 14.2 +/- 3.5 nmol/L, p = 0.004, and 18.7 +/- 0.9 nmol/L, p = 0.002, respectively). Only malondialdehyde was increased in patients compared with smoking control subjects (35.6 +/- 4.0 nmol/L, p = 0.0007). In conclusion, different classes of aldehydes were identified in exhaled breath condensate of humans. Whereas all aldehydes but nonanal were lower in control subjects as compared with other groups, only malondialdehyde distinguished smoking control subjects from patients with chronic obstructive pulmonary disease and could be envisaged as a biomarker potentially useful to monitor the disease and its response to therapy.
引用
收藏
页码:1380 / 1386
页数:7
相关论文
共 50 条
[31]   INCREASE IN CIRCULATING PRODUCTS OF LIPID-PEROXIDATION (F-2-ISOPROSTANES) IN SMOKERS - SMOKING AS A CAUSE OF OXIDATIVE DAMAGE [J].
MORROW, JD ;
FREI, B ;
LONGMIRE, AW ;
GAZIANO, JM ;
LYNCH, SM ;
SHYR, Y ;
STRAUSS, WE ;
OATES, JA ;
ROBERTS, LJ .
NEW ENGLAND JOURNAL OF MEDICINE, 1995, 332 (18) :1198-1203
[32]   Collection and analysis of exhaled breath condensate in humans [J].
Mutlu, GM ;
Garey, KW ;
Robbins, RA ;
Danziger, LH ;
Rubinstein, I .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2001, 164 (05) :731-737
[33]   Increased content of thiobarbituric acid-reactive substances and hydrogen peroxide in the expired breath condensate of patients with stable chronic obstructive pulmonary disease: no significant effect of cigarette smoking [J].
Nowak, D ;
Kasielski, M ;
Antczak, A ;
Pietras, T ;
Bialasiewicz, P .
RESPIRATORY MEDICINE, 1999, 93 (06) :389-396
[34]   Exhalation of H2O2 and thiobarbituric acid reactive substances (TBARS) by healthy subjects [J].
Nowak, D ;
Kalucka, S ;
Bialasiewicz, P ;
Król, M .
FREE RADICAL BIOLOGY AND MEDICINE, 2001, 30 (02) :178-186
[35]   Partial reversibility of airflow limitation and increased exhaled NO and sputum eosinophilia in chronic obstructive pulmonary disease [J].
Papi, A ;
Romagnoli, M ;
Baraldo, S ;
Braccioni, F ;
Guzzinati, I ;
Saetta, M ;
Ciaccia, A ;
Fabbri, LM .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2000, 162 (05) :1773-1777
[36]   Exhaled ethane, a marker of lipid peroxidation, is elevated in chronic obstructive pulmonary disease [J].
Paredi, P ;
Kharitonov, SA ;
Leak, D ;
Ward, S ;
Cramer, D ;
Barnes, PJ .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 2000, 162 (02) :369-373
[37]  
Pauwels Romain A., 2001, American Journal of Respiratory and Critical Care Medicine, V163, P1256
[38]  
PINAMONTI S, 1991, FREE RADIC BIOL MED, V25, P771
[39]   Disease of the airways in chronic obstructive pulmonary disease [J].
Piqueras, MGC ;
Cosio, MG .
EUROPEAN RESPIRATORY JOURNAL, 2001, 18 :41S-49S
[40]   Chronic obstructive pulmonary disease is associated with an increase in urinary levels of isoprostane F2αIII, an index of oxidant stress [J].
Praticò, D ;
Basili, S ;
Vieri, M ;
Cordova, C ;
Violi, F ;
Fitzgerald, GA .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1998, 158 (06) :1709-1714