Electrospinning of gelatin fibers and gelatin/PCL composite fibrous scaffolds

被引:874
作者
Zhang, YZ
Ouyang, HW
Lim, CT
Ramakrishna, S
Huang, ZM
机构
[1] Natl Univ Singapore, Div Bioengn, Singapore 117576, Singapore
[2] Natl Univ Singapore, Dept Mech Engn, Singapore 117576, Singapore
[3] Natl Univ Singapore, Dept Orthoped Surg, Singapore 119274, Singapore
[4] Natl Univ Singapore, Nanosci & Nanotechnol Initiat, Singapore 117576, Singapore
[5] Tongji Univ, Sch Aeronaut Astronaut & Mech, Shanghai 200092, Peoples R China
关键词
gelatin; electrospinning; bone-marrow stromal cells; composite nanofibrous scaffolds; tissue engineering;
D O I
10.1002/jbm.b.30128
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
In this article, ultrafine gelatin (Gt) fibers were successfully produced with the use of the electrical spinning or electrospinning technique. A fluorinated alcohol of 2,2,2-trifluoroethanol (TFE) was used as the dissolving solvent. The morphology of the electrospun gelatin fibers was found to be dependent on the alteration of gelatin concentration ranging from 2.5% w/v to 12.5% w/v at 2.5% increment intervals. Based on the electrospun gelatin fibers obtained, 10% w/v gelatin/TFE solution was selected and mixed with 10% w/v poly(is an element of-caprolactone) (PCL) in TFE at a ratio of 50:50 and co-electrospun to produce gelatin/PCL composite membranes. Contact-angle measurement and tensile tests indicated that the gelatin/ PCL complex fibrous membrane exhibited improved mechanical properties as well as more favorable wettability than that obtained from either gelatin or PCL alone. The gelatin/PCL fibrous membranes were further investigated as a promising scaffold for bone-marrow stromal cell (BMSC) culture. Scanning electron microscopy (SEM) and laser confocal microscopy observations showed that the cells could not only favorably attach and grow well on the surface of these scaffolds, but were also able to migrate inside the scaffold up to 114 mum within I week of culture. These results suggest the potential of using composite gelatin/PCL fibrous scaffolds for engineering three-dimensional tissues. (C) 2004 Wiley Periodicals, Inc.
引用
收藏
页码:156 / 165
页数:10
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