Characterisation of the ATP-dependent phosphofructokinase gene family from Arabidopsis thaliana

被引：67

作者：

Mustroph, Angelika

Sonnewald, Uwe

Blemelt, Sophia

机构：

[1] Univ Erlangen Nurnberg, Dept Biochem, D-91058 Erlangen, Germany

[2] Humboldt Univ, Inst Biol, AG Plant Physiol, D-10115 Berlin, Germany

来源：

FEBS LETTERS | 2007年 / 581卷 / 13期

关键词：

phosphofructokinase; agrobacterium infiltration; arabidopsis thaliana;

D O I：

10.1016/j.febslet.2007.04.060

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Plants possess two different types of phosphofructokinases, an ATP-dependent (PFK) and a pyrophosphate-dependent form (PFP). While plant PFPs have been investigated in detail, cDNA clones coding for PFK have not been identified in Arabidopsis thaliana. Searching the A. thaliana genome revealed 11 putative members of a phosphofructokinase gene family. Among those, four sequences showed high homology to the alpha- or beta-subunits of plant PFPs. Seven cDNAs resulted in elevated PFK, but not PFP activity after transient expression in tobacco leaves suggesting that they encode Arabidopsis PFKs. RT-PCR revealed different tissue-specific expression of the individual forms. Furthermore, analysis of GFP fusion proteins indicated their presence in different sub-cellular compartments. (c) 2007 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：2401 / 2410

页数：10

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