Chemiluminescence flow sensor for the determination of vitamin B12

被引：41

作者：

Qin, W

Zhang, ZJ ^{[1
]}

Liu, HJ

机构：

[1] Shaanxi Normal Univ, Dept Chem, Xian 710062, Peoples R China

[2] Nanjing Univ, Dept Chem, Nanjing 210008, Peoples R China

来源：

ANALYTICA CHIMICA ACTA | 1997年 / 357卷 / 1-2期

基金：

中国国家自然科学基金;

关键词：

chemiluminescence; flow sensor; vitamin B-12;

D O I：

10.1016/S0003-2670(97)00546-1

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel chemiluminescence (CL) sensor for vitamin B-12 combined with flow-injection analysis is presented in this paper. It is based on the catalytic effect of cobalt(II), liberated from vitamin B-12 by acidification, on the CL reaction between luminol, immobilized electrostatically on an anion-exchange column, and hydrogen peroxide electrochemically generated on-line via a negatively-biased electrode from dissolved oxygen in the flow cell. The sensor responds linearly to vitamin B-12 concentration in the 1.0 x 10(-3)-10 mg l(-1) range, and the detection limit is 3.5 x 10(-4) mg l(-1) vitamin B-12. A complete analysis, including sampling and washing, could be performed in 1 min with a relative standard deviation of < 3.58. The system is stable for over 500 determinations and has been applied successfully to the determination of vitamin B-12 in pharmaceutical preparations.

引用

页码：127 / 132

页数：6

共 27 条

[1] DETERMINATION OF VITAMIN-B12 AS COBALT BY ELECTROTHERMAL ATOMIC-ABSORPTION SPECTROMETRY USING THE SOLID SAMPLING TECHNIQUE [J].