Lymphoid follicle-dense mucosa at the terminal rectum is the principal site of colonization of enterohemorrhagic Escherichia coli O157:H7 in the bovine host

被引:411
作者
Naylor, SW
Low, JC
Besser, TE
Mahajan, A
Gunn, GJ
Pearce, MC
McKendrick, IJ
Smith, DGE
Gally, DL
机构
[1] Univ Edinburgh, Zoonot & Anim Pathogens Res Lab, Edinburgh EH8 9AG, Midlothian, Scotland
[2] Scottish Agr Coll, Vet Ctr, Penicuik EH26 0QE, Midlothian, Scotland
[3] Scottish Agr Coll, Vet Sci Div, Inverness IV2 4JZ, Scotland
[4] Univ Edinburgh, Ctr Trop Vet Med, Roslin EH25 9RG, Midlothian, Scotland
[5] Biomath & Stat Scotland, Edinburgh, Midlothian, Scotland
[6] Washington State Univ, Dept Vet Microbiol & Pathol, Pullman, WA 99164 USA
关键词
D O I
10.1128/IAI.71.3.1505-1512.2003
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Escherichia coli O157:117 causes bloody diarrhea and potentially fatal systemic sequelae in humans. Cattle are most frequently identified as the primary source of infection, and E. coli O157:117 generally colonizes the gastrointestinal tracts of cattle without causing disease. In this study, persistence and tropism were assessed for four different E. coli O157:117 strains. Experimentally infected calves shed the organism for at least 14 days prior to necropsy. For the majority of these animals, as well as for a naturally colonized animal obtained from a commercial beef farm, the highest numbers of E. coli O157:117 were found in the feces, with negative or significantly lower levels detected in lumen contents taken from the gastrointestinal tract. Detailed examination demonstrated that in these individuals the majority of tissue-associated bacteria were adherent to mucosal epithelium within a defined region extending up to 5 cm proximally from the recto-anal junction. The tissue targeted by E. coli O157:117 was characterized by a high density of lymphoid follicles. Microcolonies of the bacterium were readily detected on the epithelium of this region by immunofluorescence microscopy. As a consequence of this specific distribution, E. coli O157:117 was present predominately on the surface of the fecal stool. In contrast, other E. coli serotypes were present at consistent levels throughout the large intestine and were equally distributed in the stool. This is a novel tropism that may enhance dissemination both between animals and from animals to humans. The accessibility of this site may facilitate simple intervention strategies.
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页码:1505 / 1512
页数:8
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