Insertion of a SNS-specific tetrapeptide in S3-S4 linker of D4 accelerates recovery from inactivation of skeletal muscle voltage-gated Na channel mu 1 in HEK293 cells

被引:41
作者
DibHajj, SD
Ishikawa, K
Cummins, TR
Waxman, SG
机构
[1] YALE UNIV,SCH MED,DEPT NEUROL,NEW HAVEN,CT 06510
[2] VET ADM MED CTR,NEUROSCI RES CTR,W HAVEN,CT 06516
关键词
voltage dependence; Na current kinetics; channel repriming; TTX;
D O I
10.1016/S0014-5793(97)01154-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Na channel subunits alpha SNS (PN3) and alpha mu 1(SkM1) produce slowly inactivating/TTX-resistant and rapidly inactivating/TTX-sensitive currents, respectively, alpha SNS (PN3) current recovers from inactivation (reprimes) rapidly. Sequence alignment identified the tetrapeptide SLEN, in the S3-S4 linker of D4, as alpha SNS-specific. To determine whether SLEN endows Na channels with slow kinetics and/or rapid repriming, we analyzed the transient Na current produced by a chimera mu 1SLEN in HEK293 cells, Neither kinetics nor voltage dependence of activation and inactivation was affected. However, repriming was twice as fast as in the wild type at -100 mV. This suggests that SLEN may contribute to the rapid repriming of TTX-resistant Na current. (C) 1997 Federation of European Biochemical Societies.
引用
收藏
页码:11 / 14
页数:4
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